Immunohistochemistry of apoptosis-related proteins in retinoblastoma
| Autor: | Antonio Campos, Karina Braga Ribeiro, Fernando Augusto Soares, Renato José Mendonça Natalino, Célia Beatriz Gianotti Antoneli |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Retinal Neoplasms Apoptosis Pathology and Forensic Medicine 03 medical and health sciences Puma medicine Humans Cell Cycle Protein Tissue microarray biology Retinoblastoma Retinoblastoma protein Infant Cell Biology medicine.disease biology.organism_classification Immunohistochemistry Molecular biology 030104 developmental biology Child Preschool Cancer research biology.protein Mdm2 Female biological phenomena cell phenomena and immunity Apoptosis Regulatory Proteins |
| Zdroj: | Pathology - Research and Practice. 212:1144-1150 |
| ISSN: | 0344-0338 |
| DOI: | 10.1016/j.prp.2016.09.010 |
| Popis: | Retinoblastoma is the most common intraocular malignant neoplasia during childhood and results from the partial or total inactivity of the retinoblastoma protein (pRb). In the absence of pRb, the E2F transcription factors increase the levels of cell cycle proteins as well as some pro-apoptotic proteins. We intended to study the immunohistochemistry profile of apoptotic-related proteins in retinoblastoma. We also evaluated the association between the expression of apoptotic protein and stage of tumor or survivor after a 5year follow up. Apoptosis-related proteins (Apaf-1, Bak, Bax, Bcl-2, Bcl-xL, Bim-long, MDM2, p53, pro-caspase-3, PUMA, Smac/DIABLO and cleaved caspase-3) were evaluated using immunohistochemistry on tissue microarrays which contained samples of retinoblastoma tumors taken from ninety-three patients without any treatment previous to surgery. The immunohistochemistry reactions were evaluated using an optical microscope as well as the ACIS III® platform. The pro-apoptotic proteins (APAF-1, Bax, p53, PUMA, Smac/DIABLO) were more frequently expressed than the anti-apoptotic proteins (Bcl-2, Bcl-xL and MDM2). The protein Bcl-xL had a negative correlation with cleaved caspase-3, a marker of cell apoptosis. Bcl-xL may be implicated in an apoptosis block. |
| Databáze: | OpenAIRE |
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