Aroclor 1254 inhibits vasotocinergic pathways related to osmoregulatory and stress functions in the gilthead sea bream (Sparus aurata, Linnaeus 1758)
| Autor: | Juan Miguel Mancera, Juan Antonio Martos-Sitcha, Gonzalo Martínez-Rodríguez, Arleta K. Skrzynska, Magdalena Gozdowska, Ewa Kulczykowska |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Ministerio de Ciencia e Innovación (España) |
| Rok vydání: | 2019 |
| Předmět: |
Fish Proteins
Stress system Vasotocinergic pathway medicine.medical_specialty Hydrocortisone Osmotic shock Health Toxicology and Mutagenesis Hypothalamus Stimulation 010501 environmental sciences Aquatic Science 01 natural sciences 03 medical and health sciences Osmoregulation Desensitization (telecommunications) Stress Physiological Sparus aurata Internal medicine Gene expression Aroclor 1254 medicine Animals 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Kidney Osmotic concentration Chemistry Hyperosmotic transfer Chlorodiphenyl (54% Chlorine) Sea Bream medicine.anatomical_structure Endocrinology Gene Expression Regulation Liver Endocrine disruptor Pituitary Gland Water Pollutants Chemical |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0166-445X |
| Popis: | The present study assesses the response of vasotocinergic system in the gilthead sea bream (Sparus aurata)after administering two doses of the polychlorinated biphenyl Aroclor 1254 (15 or 50 μg g fresh body mass). Seven days post-administration, eight fish of each experimental group were sampled, and the remaining animals were challenged with a hyperosmotic stress by being transferred from seawater (36 ppt)to high salinity water (55 ppt)and being sampled 3 days post-transfer. Aroclor 1254 affected gene expression of avt, together with Avt concentrations in pituitary and plasma, inhibiting the stimulation observed in vasotocinergic system after hyperosmotic challenge. This was noted by the accumulation of Avt at hypophyseal level as well as by its undetectable values in plasma. Hyperosmotic transfer significantly changed branchial avtrv1a, avtrv2, atp1a and cftr mRNA expression levels in control fish, while in Aroclor 1254-treated fish they remained mostly unchanged. This desensitization also occurred for avtrs in hypothalamus, caudal kidney and liver. In addition, an enhancement in plasma cortisol concentration, together with the orchestration of several players of the Hypothalamic-Pituitary-Interrenal axis (crh, crhbp, trh, star), was also observed mostly at the highest dose used (50 μg g body mass), affecting plasma and hepatic metabolites. Our results demonstrated that Aroclor 1254 compromises the hypoosmoregulatory function of vasotocinergic system in S. aurata, also inducing a concomitant stress response. In summary, this study demonstrates that Aroclor 1254 can be considered an important endocrine disruptor in relation with the correct arrangement of vasotocinergic, metabolic and stress pathways after their stimulation by transfer to hyperosmotic environments. This study was funded by projects AGL2010-14876 (Spanish Ministry of Science and Innovation) and AGL2013-48835-C2-1-R (Spanish Ministry of Economy and Competitiveness, MINECO) awarded to J.M.M. J.A.M-S was funded by a Postdoctoral Research Fellow (Juan de la Cierva-Formación, Reference FJCI-2014-20161) from MINECO. |
| Databáze: | OpenAIRE |
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