Mechanism of tuberculostasis in mammalian serum. II. Induction of serum tuberculostasis in guinea pigs
| Autor: | Joann A. Bukovic, Ivan Kochan, Carole A. Golden |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Lipopolysaccharide Iron Guinea Pigs Infection and Immunity Biology In Vitro Techniques Microbiology Mycobacterium Guinea pig chemistry.chemical_compound Blood serum Immunity Cell Wall Internal medicine medicine Escherichia coli Animals Humans Molecular Biology chemistry.chemical_classification medicine.diagnostic_test Polysaccharides Bacterial Transferrin Culture Media Deferoxamine Endocrinology chemistry Blood chemistry Serum iron BCG Vaccine medicine.drug |
| Zdroj: | Journal of bacteriology. 100(1) |
| ISSN: | 0021-9193 |
| Popis: | The growth of tubercle bacilli in serum samples of untreated animals depends upon the availability of ionic iron which serves as a growth factor in supporting bacillary multiplication. The amount of available iron in serum is determined by the ratio between iron-saturated and iron-free transferrin; a low value for the ratio is associated with tuberculostasis (e.g., human serum, 0.4), whereas a high value is associated with the growth-supporting quality (e.g., guinea pig serum, 5.6). The treatment of guinea pigs with lipopolysaccharide ofEscherichia colior tuberculous cell wall material consistently and significantly reduced serum iron levels; a similar but less striking effect was observed in BCG-vaccinated animals. Pronounced differences were observed in the time of appearance and duration of serum hypoferremia; in lipopolysaccharide-treated animals, it appeared in 1 day and lasted for several days, whereas in BCG-vaccinated animals it appeared in about 2 weeks and lasted for much longer time periods. The induced hypoferremia was always associated with the concomitant development of serum tuberculostasis which could be neutralized by the addition of iron. These results indicate, therefore, that the mechanism of induced serum tuberculostasis in lipopolysaccharide- or tuberculous cell wall-treated and BCG-vaccinated guinea pigs is the same as that present in tuberculostatic sera of untreated animals. |
| Databáze: | OpenAIRE |
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