Mechanism of M-cell differentiation accelerated by proliferation of indigenous bacteria in rat Peyer’s patches
| Autor: | Youhei Mantani, Junichi Kawano, Hiroshi Kitagawa, Hideto Yuasa, Masaya Arai, Natsumi Masuda, Nobuhiko Hoshi, Hiroki Tsuruta, Toshifumi Yokoyama, Miho Nishida |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Brush border 040301 veterinary sciences Cellular differentiation 0403 veterinary science Peyer's Patches 03 medical and health sciences Intestinal mucosa medicine Animals rat Intestinal Mucosa Rats Wistar indigenous bacteria Peyer's patch Receptor Peyer’s patch Bacteria Full Paper General Veterinary biology Chemistry RANK Ligand M-cell differentiation Cell Differentiation Epithelial Cells 04 agricultural and veterinary sciences Molecular biology Epithelium Specific Pathogen-Free Organisms Toll-Like Receptor 4 030104 developmental biology medicine.anatomical_structure Lymphatic system RANKL immunohistochemistry Immunology biology.protein Anatomy |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | The mechanism by which indigenous bacteria on the follicle-associated epithelium (FAE) of lymphatic follicles (LFs) accelerate the differentiation of microvillous columnar epithelial cells (MV) into M-cells was immunohistochemically investigated in rat Peyer's patches. The results showed that the number of Toll-like receptor (TLR) -4(+) M-cells was greater in the FAE with expansion of bacterial colonies (LFs with bacterial colonies on the FAE: b-LF) than the FAE without expansion of bacterial colonies (nb-LF). TLR-4 was also expressed in the striated borders of MV upstream next to M-cells in the FAE of the b-LF. TLR-4(+) vesicles were frequently detected in the cytoplasms of MV with TLR-4(+) striated borders upstream next to TLR-4(+) M-cells in the FAE of b-LF. These findings suggest that TLR-4(+) MV take up TLR-4 ligands and differentiate into M-cells in the b-LF. Neither the distribution of RANK nor that of RANKL was coincident with that of M-cells in the b-LF. Moreover, RANK, but not RANKL, was expressed in intestinal villi, whereas cleaved caspase-3 was immunonegative in the MV and M-cells of the FAE, unlike in villous epithelial cells. Therefore, RANK/RANKL signaling in the LF might contribute to the down-regulation of epithelial apoptosis to facilitate the differentiation of MV into M-cells in rat Peyer's patches. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|