Cytotoxicity, interaction with dentine and efficacy on multispecies biofilms of a modified salt solution intended for endodontic disinfection in a newin vitrobiofilm model
| Autor: | S. V. van der Waal, Nina Scheres, J. J. de Soet, Paul R. Wesselink, Wim Crielaard |
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| Přispěvatelé: | Endodontologie (OII, ACTA), Preventieve tandheelkunde (OII, ACTA), Endodontology, Preventive Dentistry |
| Rok vydání: | 2014 |
| Předmět: |
Materials science
Sodium Hypochlorite Root canal Colony Count Microbial Tetrazolium Salts In Vitro Techniques Enterococcus faecalis Microbiology chemistry.chemical_compound In vivo medicine Humans General Dentistry Cells Cultured Root Canal Irrigants biology Chlorhexidine Biofilm Fibroblasts biochemical phenomena metabolism and nutrition biology.organism_classification Antimicrobial digestive system diseases Disinfection Solutions medicine.anatomical_structure chemistry Biofilms Sodium hypochlorite Dentin Anti-Infective Agents Local Dental Pulp Cavity Ex vivo medicine.drug |
| Zdroj: | International Endodontic Journal, 48(2), 153-161. Wiley-Blackwell van der Waal, S V, Scheres, N, de Soet, J J, Wesselink, P R & Crielaard, W 2014, ' Cytotoxicity, interaction with dentine and efficacy on multispecies biofilms of a modified salt solution intended for endodontic disinfection in a new in vitro biofilm model ', International Endodontic Journal, vol. 48, no. 2, pp. 153-161 . https://doi.org/10.1111/iej.12294 |
| ISSN: | 0143-2885 |
| DOI: | 10.1111/iej.12294 |
| Popis: | AimTo investigate the cytotoxicity of a modified salt solution (MSS) and evaluate the antimicrobial properties of MSS on in vitro biofilm models.MethodologyIn a metabolic assay, fibroblasts derived from periodontal ligaments (PDL) of human extracted teeth were cultured and challenged with MSS or controls. Then, in active attachment biofilm models, the efficacy of MSS in the presence of dentine powder and in eliminating mature biofilms was investigated. In the dentine assay, a biofilm of Enterococcus faecalis was employed. For the final assay, microorganisms were retrieved from infected root canals and cultured to produce biofilms. After the treatments with MSS or the controls, the biofilms were collected, serially diluted and plated. The colony-forming units were counted. One-way anova was used to analyse the differences between the groups. A P < 0.05 was considered significant.ResultsThe PDL fibroblasts remained metabolically active after challenges with MSS. Dentine powder did not alter the efficacy of MSS (P > 0.05). In endodontic biofilms, the culturable bacteria were equally reduced by MSS, 2% chlorhexidine (CHX) or 2% sodium hypochlorite (NaOCl) (P > 0.05).ConclusionsModified salt solution is noncytotoxic in vitro and has good antimicrobial properties equal to CHX and NaOCl. Although the results are promising, ex vivo and in vivo studies are needed before its use as an interappointment root canal dressing can be considered. |
| Databáze: | OpenAIRE |
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