Regulatory noncoding and predicted pathogenic coding variants of ccr5 predispose to severe covid-19
| Autor: | Vito Alessandro Lasorsa, Biancamaria Pierri, Barbara Eleni Rosato, Giulia Frisso, Giuseppe Russo, Matteo Della Monica, Roberta Russo, Pellegrino Cerino, Giuseppe Servillo, Giuseppe D'Alterio, Sueva Cantalupo, Gian Marco Cassese, Mario Capasso, Massimo Zollo, Pasquale Abete, Carlo Buonerba, Ivan Gentile, Immacolata Andolfo, Carmelo Piscopo, Giuseppe Fiorentino, Achille Iolascon |
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| Přispěvatelé: | Cantalupo, S., Lasorsa, V. A., Russo, R., Andolfo, I., D'Alterio, G., Rosato, B. E., Frisso, G., Abete, P., Cassese, G. M., Servillo, G., Gentile, I., Piscopo, C., Monica, M. D., Fiorentino, G., Russo, G., Cerino, P., Buonerba, C., Pierri, B., Zollo, M., Iolascon, A., Capasso, M. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Genotype
Receptors CCR5 QH301-705.5 SNP Single-nucleotide polymorphism Locus (genetics) Genome-wide association study Bronchi Biology Polymorphism Single Nucleotide Catalysis Article Inorganic Chemistry Cohort Studies CCR5 Exome Sequencing Databases Genetic Humans GWAS Chromosomes Human Genetic Predisposition to Disease Physical and Theoretical Chemistry Allele Biology (General) Molecular Biology Gene QD1-999 Lung Spectroscopy Exome sequencing Alleles Genetic association Genetics SARS-CoV-2 Organic Chemistry Whole exome sequencing COVID-19 Computational Biology General Medicine Computer Science Applications Chemistry Cohort Studie Genome-Wide Association Study Human |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 10 International Journal of Molecular Sciences, Vol 22, Iss 5372, p 5372 (2021) |
| Popis: | Genome-wide association studies (GWAS) found locus 3p21.31 associated with severe COVID-19. CCR5 resides at the same locus and, given its known biological role in other infection diseases, we investigated if common noncoding and rare coding variants, affecting CCR5, can predispose to severe COVID-19. We combined single nucleotide polymorphisms (SNPs) that met the suggestive significance level (P ≤ 1 × 10−5) at the 3p21.31 locus in public GWAS datasets (6406 COVID-19 hospitalized patients and 902,088 controls) with gene expression data from 208 lung tissues, Hi-C, and Chip-seq data. Through whole exome sequencing (WES), we explored rare coding variants in 147 severe COVID-19 patients. We identified three SNPs (rs9845542, rs12639314, and rs35951367) associated with severe COVID-19 whose risk alleles correlated with low CCR5 expression in lung tissues. The rs35951367 resided in a CTFC binding site that interacts with CCR5 gene in lung tissues and was confirmed to be associated with severe COVID-19 in two independent datasets. We also identified a rare coding variant (rs34418657) associated with the risk of developing severe COVID-19. Our results suggest a biological role of CCR5 in the progression of COVID-19 as common and rare genetic variants can increase the risk of developing severe COVID-19 by affecting the functions of CCR5. |
| Databáze: | OpenAIRE |
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