Enhancing β-Carotene Concentration in Parental Lines of CO6 Maize Hybrid Through Marker-Assisted Backcross Breeding (MABB)
| Autor: | Vellaikumar Sampathrajan, Jagadeeshselvam Nallathambi, Thirusenduraselvi Duraisamy, Ganesan Kalipatty Nalliappan, Senthil Natesan, Ravikesavan Rajasekaran, Bharathi Pukalenthy, Nepolean Thirunavukkarasu, Samuel Jeberson Muniyandi, Dhasarathan Manickam, Laishram Joykumar Meitei, Karthikeyan Adhimoolam, Sarankumar Chandran |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Endocrinology Diabetes and Metabolism crtRB1 Biofortification 030209 endocrinology & metabolism lcsh:TX341-641 β-Carotene Biology 03 medical and health sciences 0302 clinical medicine Genotype medicine Cultivar Carotenoid Nutrition Original Research Hybrid Co6 maize hybrid chemistry.chemical_classification 030109 nutrition & dietetics Nutrition and Dietetics business.industry MABB medicine.disease Background selection Biotechnology Vitamin A deficiency chemistry Backcrossing vitamin A deficiency business lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Frontiers in Nutrition, Vol 7 (2020) Frontiers in Nutrition |
| ISSN: | 2296-861X |
| Popis: | Vitamin A deficiency (VAD) is a global health problem; many people around the world, especially children and pregnant women, are VAD deficient or insufficient. Maize is known as an important source of provitamin A for humans. Hence, enhancement of provitamin A carotenoids (pVAC) in maize varieties through breeding or biofortification is a good option for alleviating VAD in developing countries, especially India. So far, numerous maize hybrids have been developed in India. Among them, CO6, derived from UMI1200 × UMI1230, is a popular maize hybrid and adapted to different agro-climatic zones of India, especially Tamil Nadu, a southern state of India. However, CO6 is deficient for pVAC carotenoid β-carotene. Thus, the objectives of this study were to increase the β-carotene concentration in UMI1200 and UMI1230 and generate the β-carotene enriched hybrids through marker-assisted backcross breeding (MABB). For this purpose, the maize genotype HP467-15 was used as the donor for transferring the β-carotene gene, crtRB1, into UMI1200 and UMI1230. In the MABB scheme, we used one gene-specific marker (crtRB1 3′TE) and 214 simples sequence repeat (SSR) markers for foreground and background selection, respectively. As a result, six improved lines with recurrent parent genome recovery (RPGR) ranging from 90.24 to 92.42%, along with good agronomic performance, were generated. The β-carotene concentration of the improved lines ranged from 7.056 to 9.232 μg/g. Furthermore, five hybrid combinations were generated using improved lines and evaluated in a comparative yield trial (CYT) and multi-location trials (MLT) along with the original hybrid CO6 and commercial hybrids. It was revealed that ACM-M13-002 was a superior hybrid with a 7.3-fold increase in β-carotene concentration and with a comparable yield to CO6. In summary, the improved maize inbreds can be used as possible donors for the development of β-carotene-rich cultivars in maize breeding programs and the β-carotene enriched hybrid developed in this study will hold great promise for food and nutritional security. |
| Databáze: | OpenAIRE |
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