Semen leukocytes and oxidative-dependent DNA damage of spermatozoa in male partners of subfertile couples with no symptoms of genital tract infection

Autor:	Felice Francavilla, Giuliana Cordeschi, Stefano Necozione, Settimio D'Andrea, Sandro Francavilla, Arcangelo Barbonetti, A. Micillo, M. R. C. Vassallo
Rok vydání:	2016
Předmět:	Adult Male 0301 basic medicine endocrine system subfertility DNA damage Urology Endocrinology Diabetes and Metabolism Apoptosis Semen DNA Fragmentation Biology Semen analysis Reproductive Tract Infections Male infertility Andrology 03 medical and health sciences Semen quality 0302 clinical medicine Endocrinology Leukocytes medicine Humans seminal leukocytes Infertility Male 8-hydroxy-20-deoxyguanosine DNA fragmentation seminal leukocytes spermatozoa subfertility 030219 obstetrics & reproductive medicine medicine.diagnostic_test urogenital system Age Factors medicine.disease Spermatozoa Sperm 8-hydroxy-20-deoxyguanosine Semen Analysis Oxidative Stress 030104 developmental biology Reproductive Medicine Terminal deoxynucleotidyl transferase DNA fragmentation Female DNA Damage
Zdroj:	Andrology. 4:808-815
ISSN:	2047-2919
DOI:	10.1111/andr.12188
Popis:	The influence of seminal leukocytes on generation of oxidative damage to sperm DNA was here investigated on male partners of subfertile couples asymptomatic for a genital tract infection. The study included 111 ejaculates from men attending the Andrology Centre at University of L'Aquila. Semen leukocytes subset included round cells expressing pan-leukocyte CD45 antigen, monocyte/macrophage lineage antigen CD14, and activated macrophages HLA-DR antigen. The 8-hydroxy-2'-deoxyguanosine (8-OHdG) expression identified spermatozoa with DNA oxidative adducts while terminal deoxynucleotidyl transferase (TdT)-mediated fluorescein-dUTP nick end labeling (TUNEL) assay detected spermatozoa with DNA fragmentation. Flow cytometry and immunocytochemistry was used for determinations. Main outcome measure was the association of semen leukocyte subpopulations with spermatozoa showing oxidative-related DNA damage and with routine semen parameters. Leukocyte subpopulations were strictly correlated (p
Databáze:	OpenAIRE
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