A FRET‐Based Near‐Infrared Fluorescent Probe for Ratiometric Detection of Cysteine in Mitochondria
| Autor: | Yibin Zhang, Mingxi Fang, Thomas Werner, Shulin Wan, Tessa E. Steenwinkel, Haiying Liu, Rudy L. Luck, Shuai Xia, Tyler Phillips, Logan Mikesell |
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| Rok vydání: | 2019 |
| Předmět: |
Lipopolysaccharides
Infrared Rays 010402 general chemistry 01 natural sciences Biochemistry Article Rhodamine chemistry.chemical_compound Fluorescence Resonance Energy Transfer Animals heterocyclic compounds Cysteine Hydrogen peroxide Molecular Biology Fluorescent Dyes Molecular Structure 010405 organic chemistry Optical Imaging Organic Chemistry Hydrogen Peroxide Coumarin Acceptor Fluorescence Mitochondria 0104 chemical sciences Oxidative Stress Drosophila melanogaster Förster resonance energy transfer chemistry Biophysics Molecular Medicine Preclinical imaging |
| Zdroj: | Chembiochem |
| ISSN: | 1439-7633 1439-4227 |
| Popis: | We report a near-infrared fluorescent probe A for the ratiometric detection of cysteine based on FRET from a coumarin donor to a near-infrared rhodamine acceptor. Upon addition of cysteine, the coumarin fluorescence increased dramatically up to 18-fold and the fluorescence of the rhodamine acceptor decreased moderately by 45 % under excitation of the coumarin unit. Probe A has been used to detect cysteine concentration changes in live cells ratiometrically and to visualize fluctuations in cysteine concentrations induced by oxidation stress through treatment with hydrogen peroxide or lipopolysaccharide (LPS). Finally, probe A was successfully applied for the in vivo imaging of Drosophila melanogaster larvae to measure cysteine concentration changes. |
| Databáze: | OpenAIRE |
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