Differential dose-dependent effects of zinc oxide nanoparticles on oxidative stress-mediated pancreatic β-cell death
| Autor: | Kishore M. Paknikar, Rinku D Umrani, Swati C Asani |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Programmed cell death Antioxidant Cell Survival medicine.medical_treatment Biomedical Engineering Medicine (miscellaneous) Metal Nanoparticles Bioengineering Apoptosis 02 engineering and technology Oxidative phosphorylation Development medicine.disease_cause Antioxidants Cell Line 03 medical and health sciences chemistry.chemical_compound Insulin-Secreting Cells medicine Animals Humans Hypoglycemic Agents General Materials Science Particle Size chemistry.chemical_classification Reactive oxygen species Super oxide dismutase Chemistry Superoxide Dismutase Glutathione Hydrogen Peroxide 021001 nanoscience & nanotechnology Molecular biology Endocytosis Rats Oxidative Stress 030104 developmental biology Biochemistry Zinc Oxide 0210 nano-technology Reactive Oxygen Species Oxidation-Reduction Oxidative stress |
| Zdroj: | Nanomedicine (London, England). 12(7) |
| ISSN: | 1748-6963 |
| Popis: | Aim: To study the effects of zinc oxide nanoparticles (ZON) on oxidative stress-mediated pancreatic β-cell death. Methods: Cellular uptake of ZON, effects on antioxidant factors and apoptosis were studied. Results: ZON get internalized by endocytosis and increase intracellular zinc ion levels. ZON treatment (30 and 100 μg/ml) to RIN5f cells resulted in cytotoxicity, oxidative stress and apoptosis. ZON (1, 3, 10 μg/ml, subcytotoxic concentrations) increased super oxide dismutase activity and levels of reduced glutathione in RIN5f cells. Furthermore, ZON (subcytotoxic concentrations) protected RIN5f cells from H2O2-induced oxidative stress as evidenced by reduced reactive oxygen species levels; increased super oxide dismutase activity and glutathione levels; and reduced apoptotic death. Conclusion: ZON (subcytotoxic concentrations) protect pancreatic β cells from oxidative-stress-mediated cell death. |
| Databáze: | OpenAIRE |
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