The alternative spliced 3'-UTR mediated differential secretion of macrophage colony stimulating factor in breast cancer cells
| Autor: | Ho Hyung Woo, Setsuko K. Chambers |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Macrophage colony-stimulating factor Biophysics Breast Neoplasms Biochemistry ELAV-Like Protein 1 03 medical and health sciences 0302 clinical medicine Cell Movement Cell Line Tumor Gene silencing Humans Secretion Gene Silencing Molecular Biology 3' Untranslated Regions Messenger RNA Chemistry Three prime untranslated region Macrophage Colony-Stimulating Factor Alternative splicing Cell Biology Cellular phenotype Cell biology Alternative Splicing 030104 developmental biology 030220 oncology & carcinogenesis Female Breast cancer cells |
| Zdroj: | Biochemical and biophysical research communications. 525(4) |
| ISSN: | 1090-2104 |
| Popis: | CSF-1 mRNA 3′UTR variants (var) are generated from alternative splicing. CSF-1 protein encoded by var-1 mRNA with long 3′UTR derived from exon-10 is rapidly secreted compared to the CSF-1 protein encoded by var-4 mRNA with short 3′UTR derived from exon-9. Secretion kinetics indicates that HuR, which binds the CSF-1 var-1 mRNA, but not var-4 mRNA, accelerates the secretion of CSF-1 protein. HuR overexpression increases the secretion rate of CSF-1 protein. In contrast, silencing of HuR does not have such an effect, suggesting other compensatory mechanisms. Effect of the CSF-1 mRNA variant 3′UTRs on cellular phenotype shows both CSF-1 var-1 or -4 mRNA is involved in the enhanced rates of migration and invasion observed by both in vitro in breast cancer cells. Our study indicates that the alternative splicing of CSF-1 mRNA 3′UTR can regulate differential secretion of CSF-1 protein. |
| Databáze: | OpenAIRE |
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