Trans-fatty acids aggravate anabolic steroid-induced metabolic disturbances and differential gene expression in muscle, pancreas and adipose tissue

Autor: Rômulo Dias Novaes, Reggiani Vilela Gonçalves, Eliziária C. Santos, Thaiany G. Souza-Silva, Etienne Guillocheau, Rafael F. Oliveira, Jamili D.B. Santos, Robson E. Silva, Natanny S. Silva
Přispěvatelé: Universidade Federal de Vicosa (UFV), Universidade Federal de Alfenas, Laboratoire de Biochimie et Nutrition Humaine, AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Universidade Federal dos Vales do Jequitinhonha e Mucuri = Federal University of Jequitinhonha and Mucuri Vallays (UFJMV)
Rok vydání: 2019
Předmět:
Blood Glucose
0301 basic medicine
medicine.medical_specialty
Anabolism
medicine.medical_treatment
Adipose tissue
Diet
High-Fat
030226 pharmacology & pharmacy
General Biochemistry
Genetics and Molecular Biology
Mice
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Internal medicine
medicine
Animals
General Pharmacology
Toxicology and Pharmaceutics
Muscle
Skeletal
Pancreas
Testosterone Congeners
ComputingMilieux_MISCELLANEOUS
Glycemic
medicine.diagnostic_test
business.industry
Pancreatic islets
Glycemic Load
Skeletal muscle
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
General Medicine
Trans Fatty Acids
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
Lipid Metabolism
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
3. Good health
Mice
Inbred C57BL
Glucose
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Adipose Tissue
Liver
Female
Insulin Resistance
Energy Metabolism
Lipid profile
business
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Anabolic steroid
Zdroj: Life Sciences
Life Sciences, Elsevier, 2019, 232, pp.116603. ⟨10.1016/j.lfs.2019.116603⟩
ISSN: 0024-3205
Popis: Aims Although anabolic steroids (AS) and trans-fatty acids overload exerts systemic toxicity and are independent risk factors for metabolic and cardiovascular disorders, their interaction remains poorly understood. Thus, we investigated the impact of a diet rich in trans-fatty acids (HFD) combined with AS on glycemic control, lipid profile, adipose tissue, skeletal muscle and pancreas microstructure and expression of genes involved in energy metabolism. Main methods Forty-eight C57BL/6 mice were randomized into 6 groups treated for 12 weeks with a standard diet (SD) or a diet rich in C18:1 trans-fatty isomers (HFD), alone or combined with 10 or 20 mg/kg testosterone cypionate (AS). Key findings Our results indicated that AS improved glycemic control, upregulated gene expression of Glut-4 and CPT-1 in skeletal muscle, FAS, ACC and UCP-1 in adipose tissue. AS also reduced total and LDL cholesterol in mice fed a SD. When combined with the HFD, AS was unable to induce microstructural adaptations in adipose tissue, pancreatic islets and β-cells, but potentiated GCK and Glut-2 (pancreas) and Glut-4 and CPT-1 (skeletal muscle) upregulation. HFD plus AS also downregulated FAS and ACC gene expression in adipose tissue. Combined with HFD, AS increased triacylglycerol circulating levels, improved insulin sensitivity and glycemic control in mice. Significance Our findings indicated that HFD and AS can interact to modulates glycemic control and lipid profile by a mechanism potentially related with a reprogramming of genes expression in organs such as the pancreas, adipose tissue and skeletal muscle.
Databáze: OpenAIRE
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