Intracellular localization of the migration inhibitory factor (MIF) in a long-term human lymphoid cell line
| Autor: | Kurt Hirschhorn, Philip R. Glade, Michael B. Prystowsky, Walter S. Ceglowski, Carol F. Sorokin |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Intracellular localization
Immunology Biology Cell Fractionation Esterase Cell Line Nonspecific esterase Culture Techniques Microsomes Immunology and Allergy Humans Infectious Mononucleosis Lymphocytes Macrophage Migration-Inhibitory Factors Glucuronidase Differential centrifugation L-Lactate Dehydrogenase Esterases General Medicine Migration Inhibitory Factor Biochemistry Cell culture Cell Migration Inhibition Microsome Specific activity Lysosomes |
| Zdroj: | International archives of allergy and applied immunology. 48(2) |
| ISSN: | 0020-5915 |
| Popis: | Subcellular fractions of the human lymphoid cell line PGLC-33H were obtained by N2 cavitation and differential centrifugation. The purity of the fractions was assessed by the use of the following marker enzymes: β-glucuronidase for the lysosomal-intermediate fraction; a nonspecific esterase for the microsomal fraction; and LDH for the supernatant fraction. These subcellular fractions were studied for MIF activity utilizing human lymphoid cells from established lines as target cells. MIF activtiy was most consistently found in the microsomal fraction. Also, MIF activity was closely associated with the relative specific activity of esterase. No such correlation with MIF activity could be demonstrated for β-glucuronidase or LDH. |
| Databáze: | OpenAIRE |
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