Substitution of Soy Protein for Casein Prevents Oxidative Modification and Inflammatory Response Induced in Rats Fed High Fructose Diet
| Autor: | Emayavaramban Priyadarshini, Krishnamoorthy Bhavani, Carani Venkatraman Anuradha, Rajagopalan Geetha, S. Sreeja |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Article Subject Adiponectin business.industry Insulin medicine.medical_treatment Fructose Oxidative phosphorylation medicine.disease_cause chemistry.chemical_compound Endocrinology Biochemistry chemistry Internal medicine Casein medicine Phosphorylation business Soy protein Oxidative stress Research Article |
| Zdroj: | ISRN Inflammation |
| ISSN: | 2090-8695 |
| DOI: | 10.1155/2014/641096 |
| Popis: | Fructose-rich diet is known to cause metabolic dysregulation, oxidative stress, and inflammation. We aimed to compare the effects of two dietary proteins of animal and plant origins on fructose-induced oxidative stress and inflammatory changes in liver. Wistar rats were fed either starch or fructose (60%) diet with casein or soy protein (20%) as the protein source for 8 weeks. Glucose and insulin, glycated hemoglobin and fructosamine, AOPP, and FRAP were determined in circulation. Intracellular ROS, oxidatively modified proteins (4-HNE and 3-NT adducts), adiponectin, TNF-α, IL-6 and PAI-1 mRNA expression, phosphorylation and activation of JNK and IKKβ, and NF-κB binding activity were assayed in liver. In comparison with starch fed group, fructose + casein group registered significant decline in antioxidant potential and increase in plasma glucose, insulin, and glycated proteins. Increased ROS production, 4-HNE and 3-NT modified proteins, JNK and IKKβ activation, and NF-κB binding activity were observed in them along with increased gene expression of PAI-1, IL-6, and TNF-α and decreased adiponectin expression. Substitution of soy protein for casein reduced oxidative modification and inflammatory changes in fructose-fed rats. These data suggest that soy protein but not casein can avert the adverse effects elicited by chronic consumption of fructose. |
| Databáze: | OpenAIRE |
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