Changes in VGLUT1 and VGLUT2 expression in rat dorsal root ganglia and spinal cord following spared nerve injury
| Autor: | Gang Yu, Hongsheng Wang, Rui-Bin Su, Ze-Hui Gong, Shou-Pu Yi, Zhi-Tong Wang |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty SNi Gene Expression Rats Sprague-Dawley Lumbar enlargement 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Sural Nerve Glutamate homeostasis Ganglia Spinal Internal medicine medicine Animals business.industry Glutamate receptor Peroneal Nerve Cell Biology Nerve injury Spinal cord Rats 030104 developmental biology medicine.anatomical_structure Endocrinology Spinal Cord Anesthesia Vesicular Glutamate Transport Protein 1 Neuropathic pain Peripheral nerve injury Vesicular Glutamate Transport Protein 2 Neuralgia Sciatic Neuropathy Tibial Nerve medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | Neurochemistry International. 99:9-15 |
| ISSN: | 0197-0186 |
| Popis: | Disturbance of glutamate homeostasis is a well-characterized mechanism of neuropathic pain. Vesicular glutamate transporters (VGLUTs) determine glutamate accumulation in synaptic vesicles and their roles in neuropathic pain have been suggested by gene-knockout studies. Here, we investigated the spatio-temporal changes in VGLUT expression during the development of neuropathic pain in wild-type rats. Spared nerve injury (SNI) induced mechanical allodynia from postoperative day 1 to at least day 14. Expression of VGLUT1 and VGLUT2 in dorsal root ganglia and spinal cord was examined by western blot analyses on different postoperative days. We observed that VGLUT2 were selectively upregulated in crude vesicle fractions from the ipsilateral lumbar enlargement on postoperative days 7 and 14, while VGLUT1 was transiently downregulated in ipsilateral DRG (day 4) and contralateral lumbar enlargement (day 1). Upregulation of VGLUT2 was not accompanied by alterations in vesicular expression of synaptotagmin or glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Thus, VGLUTs expression, especially VGLUT2, is regulated following peripheral nerve injury. Temporal regulation of VGLUT2 expression in spinal cord may represent a novel presynaptic mechanism contributing to injury-induced glutamate imbalance and associated neuropathic pain. |
| Databáze: | OpenAIRE |
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