Sub-nanowatt microfluidic single-cell calorimetry
| Autor: | Edward Dechaumphai, Renkun Chen, Ratneshwar Lal, Anne N. Murphy, Courtney R. Green, Sahngki Hong, Christian M. Metallo |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Materials science Science Microfluidics Cell General Physics and Astronomy Bioengineering 02 engineering and technology Calorimetry Noise (electronics) Characterization and analytical techniques General Biochemistry Genetics and Molecular Biology Article Cell size Tetrahymena thermophila 03 medical and health sciences Thermal conductivity Oxygen Consumption medicine Metabolomics lcsh:Science Multidisciplinary Microchannel Lab-on-a-chip Temperature Nanobiotechnology Thermal Conductivity General Chemistry Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology Mitochondria 030104 developmental biology medicine.anatomical_structure Metabolic rate Biophysics lcsh:Q Basal Metabolism Single-Cell Analysis 0210 nano-technology Biotechnology |
| Zdroj: | Nature communications, vol 11, iss 1 Nature Communications Nature Communications, Vol 11, Iss 1, Pp 1-9 (2020) |
| Popis: | Non-invasive and label-free calorimetry could become a disruptive technique to study single cell metabolic heat production without altering the cell behavior, but it is currently limited by insufficient sensitivity. Here, we demonstrate microfluidic single-cell calorimetry with 0.2-nW sensitivity, representing more than ten-fold enhancement over previous record, which is enabled by (i) a low-noise thermometry platform with ultralow long-term (10-h) temperature noise (80 μK) and (ii) a microfluidic channel-in-vacuum design allowing cell flow and nutrient delivery while maintaining a low thermal conductance of 2.5 μW K−1. Using Tetrahymena thermophila as an example, we demonstrate on-chip single-cell calorimetry measurement with metabolic heat rates ranging from 1 to 4 nW, which are found to correlate well with the cell size. Finally, we perform real-time monitoring of metabolic rate stimulation by introducing a mitochondrial uncoupling agent to the microchannel, enabling determination of the spare respiratory capacity of the cells. Calorimetrically measuring the heat of single cells is currently not possible due to the sensitivity of existing calorimeters. Here the authors present on-chip single cell calorimetry, with a sensitivity over ten-fold greater than the current gold-standard. |
| Databáze: | OpenAIRE |
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