Rapid immuno-SERS microscopy for tissue imaging with single-nanoparticle sensitivity
| Autor: | Alexander Marx, Sebastian Schlücker, Philipp Ströbel, Mohammad Salehi, Jens Packeisen, Dennis Steinigeweg |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Fluorescence-lifetime imaging microscopy Materials science Time Factors Tissue imaging Surface Properties Chemie General Physics and Astronomy Nanoparticle Metal Nanoparticles Spectrum Analysis Raman General Biochemistry Genetics and Molecular Biology symbols.namesake Nuclear magnetic resonance Microscopy Humans General Materials Science General Engineering Prostate General Chemistry Gold nanospheres Silicon Dioxide Immunohistochemistry Family member Colloidal gold symbols Gold Raman spectroscopy |
| Zdroj: | Journal of biophotonics. 6(10) |
| ISSN: | 1864-0648 |
| Popis: | Immuno-SERS microscopy is a novel imaging technique in nano-biophotonics, which employs antibodies labeled with SERS-active nanoparticles in conjunction with Raman microscopy. Rapid data acquisition is of central importance for screening large areas of tissue specimens. Here, we first discuss the role of SERS labels with single-particle sensitivity in immuno-SERS microscopy, in particular with respect to false-negative results. In combined single-particle experiments (SERS microscopy/dark-field microscopy/HR-SEM), we then demonstrate that small glass-coated clusters (dimers and trimers) of gold nanospheres exhibit the desired single-particle SERS sensitivity, even at acquisition times as short as 30 msec per pixel, while monomers do not. The proof-of-concept for rapid immuno-SERS microscopy with 30 msec acquisition time per pixel for selective imaging of the p53 family member p63 in prostate tissue sections is demonstrated. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) |
| Databáze: | OpenAIRE |
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