Antagonistic Effect of Nucleic Acid Precursors on the Anticoccidial Activity of 6-Azauracil
Autor: | Yukitaka Okada, Tsutomu Okamoto, Toshimi Matsuno, Toshikatsu Hayashi, Nobuo Kobayashi, Fumio Hariguchi |
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Rok vydání: | 1996 |
Předmět: |
Male
Adenosine Pyrimidine Drug Resistance Biology chemistry.chemical_compound Orotidine parasitic diseases medicine Animals Drug Interactions Uracil Uridine Poultry Diseases Orotic Acid Dose-Response Relationship Drug General Veterinary Coccidiosis Adenine Nucleic Acid Precursors Metabolism In vitro Biochemistry chemistry Food Fortified Nucleic acid Coccidiostats Eimeria Chickens medicine.drug |
Zdroj: | Journal of Veterinary Medical Science. 58:121-127 |
ISSN: | 1347-7439 0916-7250 |
DOI: | 10.1292/jvms.58.121 |
Popis: | The antagonistic phenomena to anticoccidial activities of 6-azauracil (AzU) induced by certain nucleic acid precursors were examined in battery experiments. Each nucleic acid precursor, orotate, orotidine, uracil, uridine, adenine and adenosine was mixed in feed in combination with effective levels of AzU. The test feed was provided through the test term to White Leghorn cockerels which were infected with Eimeria tenella, E. necatrix or E. acervulina. In vitro tests were also conducted for antagonistic phenomena similarly to in vivo test using E. tenella cultured in chick kidney (CK) cells. Orotate and uridine reduced dose-dependently the anticoccidial activity of AzU in E. tenella infection in chickens. Uracil and orotidine revealed similar effect. Adenine and adenosine did not show any antagonistic effect to AzU. In E. necatrix infection, orotate and uridine had a similar tendency to that of E. tenella, whereas uracil and orotidine was less pronounced. The activity of AzU against E. acervulina was also blocked by the treatment with uridine and orotate. AzU inhibited the schizont's development of E. tenella in the CK cells, and this effect was reduced by uridine, but not by orotate and orotidine. The antagonistic effect of the pyrimidine nucleic acid precursors on AzU activity suggested that the anticoccidial effect of AzU to E. tenella, E. necatrix and E. acervulina is derived from inhibition of the metabolism of pyrimidine nucleotide synthesis in these parasites. |
Databáze: | OpenAIRE |
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