Abscisic acid supports colonization of Eucalyptus grandis roots by the mutualistic ectomycorrhizal fungus Pisolithus microcarpus
Autor: | Anna Lipzen, Igor V. Grigoriev, Richard Hill, Donovin Coles, Krista L. Plett, Christopher I Cazzonelli, Francis Martin, Ian C. Anderson, Johanna Wong-Bajracharya, Vivian Ng, Sidra Anwar, Thomas C. Jeffries, Mei Wang, Jonathan M. Plett |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
Physiology Plant Science Fungus Plant Roots 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Metabolomics Symbiosis Mycorrhizae Botany Transcriptional regulation Colonization Gene Abscisic acid 030304 developmental biology Eucalyptus 0303 health sciences biology Host (biology) Basidiomycota fungi food and beverages 15. Life on land biology.organism_classification chemistry Abscisic Acid 010606 plant biology & botany |
Zdroj: | New Phytologist. 233:966-982 |
ISSN: | 1469-8137 0028-646X |
Popis: | The pathways regulated in ectomycorrhizal (EcM) plant hosts during the establishment of symbiosis are not as well understood when compared to the functional stages of this mutualistic interaction. Our study used the EcM host Eucalyptus grandis to elucidate symbiosis-regulated pathways across the three phases of this interaction. Using a combination of RNA sequencing and metabolomics we studied both stage-specific and core responses of E. grandis during colonization by Pisolithus microcarpus. Using exogenous manipulation of the abscisic acid (ABA), we studied the role of this pathway during symbiosis establishment. Despite the mutualistic nature of this symbiosis, a large number of disease signalling TIR-NBS-LRR genes were induced. The transcriptional regulation in E. grandis was found to be dynamic across colonization with a small core of genes consistently regulated at all stages. Genes associated to the carotenoid/ABA pathway were found within this core and ABA concentrations increased during fungal integration into the root. Supplementation of ABA led to improved accommodation of P. microcarpus into E. grandis roots. The carotenoid pathway is a core response of an EcM host to its symbiont and highlights the need to understand the role of the stress hormone ABA in controlling host-EcM fungal interactions. |
Databáze: | OpenAIRE |
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