The obligate alkalophilic soda-lake fungus Sodiomyces alkalinus has shifted to a protein diet

Autor: In Geol Choi, Byoungnam Min, Chris Daum, Anna Lipzen, Duur K. Aanen, Ronald P. de Vries, Daniel Luciano Falkoski, Igor V. Grigoriev, Bernard Henrissat, Adrian Tsang, Joost van den Heuvel, Jan A. L. van Kan, E. N. Bilanenko, Alfons J. M. Debets, Alexey A. Grum-Grzhimaylo, Claudio A. Valero-Jiménez
Přispěvatelé: Wageningen University and Research Centre [Wageningen] (WUR), Utrecht University [Utrecht], US Department of Energy Joint Genome Institute, Walnut Creek CA, USA, US Department of Energy Joint Genome Institute, U.S Department of Energy, U.S. Department of Energy (DOE)-U.S. Department of Energy (DOE), United States Department of Energy, Centre for Structural and Functional Genomics, Concordia University [Montreal], Architecture et fonction des macromolécules biologiques (AFMB), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Lomonosov Moscow State University (MSU), Laboratory of Phytopathology, Department of Energy / Joint Genome Institute (DOE), Los Alamos National Laboratory (LANL), Westerdijk Fungal Biodiversity Institute, Westerdijk Fungal Biodiversity Institute - Fungal Physiology, Sub Molecular Plant Physiology, Molecular Plant Physiology, Wageningen University and Research [Wageningen] (WUR), U.S. Department of Energy [Washington] (DOE)-U.S. Department of Energy [Washington] (DOE), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Gene Transfer
Alkalies
brine shrimps
01 natural sciences
Bacterial cell structure
prokaryotes
Phylogeny
Genome
biology
Soda Lakes
HGT
Plants
Hydrogen-Ion Concentration
Biological Sciences
PE&RC
[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM]
Fungal
Horizontal gene transfer
geography.geographical_feature
Proteome
Laboratory of Genetics
Genome
Fungal
Biotechnology
Gene Transfer
Horizontal
enzymes
Fungus
Cellulase
Laboratorium voor Erfelijkheidsleer
Horizontal
03 medical and health sciences
Ascomycota
Sodiomyces alkalinus
Botany
Genetics
Ecology
Evolution
Behavior and Systematics
Nutrition
geography
Evolutionary Biology
Obligate
alkalophilic fungus
fungi
15. Life on land
biology.organism_classification
Laboratorium voor Phytopathologie
Lakes
030104 developmental biology
Laboratory of Phytopathology
biology.protein
EPS
Bacteria
010606 plant biology & botany
Zdroj: Molecular Ecology
Molecular Ecology, Wiley, 2018, 27 (23), pp.4808-4819. ⟨10.1111/mec.14912⟩
Molecular Ecology, 27(23), 4808-4819. Wiley-Blackwell
Molecular ecology, vol 27, iss 23
Molecular Ecology, 27(23), 4808-4819
Molecular Ecology, 2018, 27 (23), pp.4808-4819. ⟨10.1111/mec.14912⟩
Molecular Ecology, 27(23), 4808. Blackwell Publishing Ltd
Molecular Ecology 27 (2018) 23
ISSN: 0962-1083
1365-294X
DOI: 10.1111/mec.14912⟩
Popis: International audience; Sodiomyces alkalinus is one of the very few alkalophilic fungi, adapted to grow optimally at high pH. It is widely distributed at the plant-deprived edges of extremely alkaline lakes and locally abundant. We sequenced the genome of S. alkalinus and reconstructed evolution of catabolic enzymes, using a phylogenomic comparison. We found that the genome of S. alkalinus is larger, but its predicted proteome is smaller and heavily depleted of both plant-degrading enzymes and proteinases, when compared to its closest plant-pathogenic relatives. Interestingly, despite overall losses, S. alkalinus has retained many proteinases families and acquired bacterial cell wall-degrading enzymes, some of them via horizontal gene transfer from bacteria. This fungus has very potent proteolytic activity at high pH values, but slowly induced low activity of cellulases and hemicellulases. Our experimental and in silico data suggest that plant biomass, a common food source for most fungi, is not a preferred substrate for S. alkalinus in its natural environment. We conclude that the fungus has abandoned the ancestral plant-based diet and has become specialized in a more protein-rich food, abundantly available in soda lakes in the form of prokaryotes and small crustaceans.
Databáze: OpenAIRE
Externí odkaz: