Immunohistochemical detection of human basophils in late-phase skin reactions
| Autor: | Anne-Marie Irani, Burton Zweiman, Ala' Nafie, Christopher L. Kepley, Han-Zhang Xia, El Desouki Fouda, Shirley S. Craig, Lawrence B. Schwartz, Candice Huang |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Pathology
medicine.medical_specialty Allergy Biopsy Immunology Tryptase Basophil Poaceae medicine.disease_cause Dermatitis Atopic Allergic inflammation Mice chemistry.chemical_compound Allergen Endopeptidases Animals Humans Immunology and Allergy Medicine Mast Cells Skin Skin Tests biology Histocytochemistry business.industry Antibodies Monoclonal Allergens medicine.disease Mast cell Immunohistochemistry Basophils Staining medicine.anatomical_structure chemistry biology.protein Blood Vessels Pollen business Histamine |
| Zdroj: | Journal of Allergy and Clinical Immunology. 101:354-362 |
| ISSN: | 0091-6749 |
| DOI: | 10.1016/s0091-6749(98)70248-9 |
| Popis: | Background: Human basophils are difficult to detect with classic histochemical stains at sites of allergic inflammation. The 2D7+ anti-basophil monoclonal antibody was used to identify basophils in skin during the late-phase response to a cutaneous allergen challenge. Methods: The 2D7+ monoclonal antibody was used on protease-digested sections of skin biopsy specimens obtained 6 and 24 hours after an allergen or buffer challenge. The skin chamber technique was used to compare buffer- and allergen-challenged sites at 6 hours, and intradermal injection of allergen was used to compare allergen-challenged sites at 6 and 24 hours. Results: Dramatic increases in the numbers of 2D7+ cells and in tissue staining by 2D7+ were observed 6 hours after allergen challenge compared with buffer challenge. Histamine levels in skin chamber fluid varied with 2D7+ cell concentrations. By 24 hours, 2D7+ cells and tissue staining appeared to diminish but were still detectable in the allergen-challenged sites. Basophils localized primarily in and around blood vessels, whereas mast cells remained mostly in the superficial dermis. Mast cells were 2D7− in both the allergen- and buffer-challenged skin. Metachromatic staining of 2D7 basophils with toluidine blue was absent in these tissue sections. Conclusions: The 2D7 monoclonal antibody provides a more sensitive and precise marker than histochemical staining for human basophil involvement during the late-phase response to an allergen challenge. Basophil infiltration was observed at 6 hours only after allergen challenge and persisted at similar levels by 24 hours. (J Allergy Clin Immunol 1998;101:354-62.) |
| Databáze: | OpenAIRE |
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