Asymmetric Stratification-Induced Polarity Loss and Coordinated Individual Cell Movements Drive Directional Migration of Vertebrate Epithelium
| Autor: | Ophir D. Klein, Yunzhe Lu, Ruolan Deng, Huanyang You, Yishu Xu, Pengfei Lu, Christopher L. Antos, Jianlong Sun |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Medical Physiology Epithelium Madin Darby Canine Kidney Cells Mice 0302 clinical medicine Cell Movement Cell polarity chemotaxis Cancer Epithelial polarity Cell Polarity Mammary Glands Cell biology Organoids cell polarity medicine.anatomical_structure Mammary Epithelium Vertebrates Female apicobasal polarity branching morphogenesis Signal Transduction Polarity (physics) Green Fluorescent Proteins epithelial-mesenchymal transition collective migration Biology General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Dogs Mammary Glands Animal medicine Animals Epithelial–mesenchymal transition FGF gradient epithelial polarity Cell Proliferation epithelial stratification front-rear polarity Animal Cell growth Chemotaxis Epithelial Cells 030104 developmental biology Cell Surface Extensions Biochemistry and Cell Biology Fibroblast Growth Factor 10 030217 neurology & neurosurgery |
| Zdroj: | Cell reports, vol 33, iss 2 Cell reports |
| Popis: | SUMMARY Collective migration is essential for development, wound repair, and cancer metastasis. For most collective systems, “leader cells” determine both the direction and the power of the migration. It has remained unclear, however, how the highly polarized vertebrate epithelium migrates directionally during branching morphogenesis. We show here that, unlike in other systems, front-rear polarity of the mammary epithelium is set up by preferential cell proliferation in the front in response to the FGF10 gradient. This leads to frontal stratification, loss of apicobasal polarity, and leader cell formation. Leader cells are a dynamic population and move faster and more directionally toward the FGF10 signal than do follower cells, partly because of their intraepithelial protrusions toward the signal. Together, our data show that directional migration of the mammary epithelium is a unique multistep process and that, despite sharing remarkable cellular and molecular similarities, vertebrate and invertebrate epithelial branching are fundamentally distinct processes. In Brief Lu et al. demonstrate that directional migration of mammary epithelium is a unique multistep process that includes asymmetric stratification, loss of apicobasal polarity, and active migration stages. Leader cells are a dynamic population, which form intra-epithelial protrusions and move faster and more directionally than follower cells do toward the signal source. Graphical Abstract |
| Databáze: | OpenAIRE |
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