Variables affecting the quantitation of CD22 in neoplastic B cells
Autor: | Robert J. Kreitman, Gregory A. Jasper, Gerald E. Marti, Indu Arun, Constance M. Yuan, Maryalice Stetler-Stevenson, David Venzon, Alan S. Wayne |
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Rok vydání: | 2010 |
Předmět: |
Pathology
medicine.medical_specialty Histology Sialic Acid Binding Ig-like Lectin 2 Chronic lymphocytic leukemia Follicular lymphoma Lymphoma Mantle-Cell Article Pathology and Forensic Medicine Flow cytometry Antigen immune system diseases hemic and lymphatic diseases medicine Humans Hairy cell leukemia Lymphoma Follicular Cell Proliferation B-Lymphocytes Leukemia Hairy Cell biology medicine.diagnostic_test Cell Biology Precursor Cell Lymphoblastic Leukemia-Lymphoma Flow Cytometry medicine.disease Leukemia Lymphocytic Chronic B-Cell Leukemia biology.protein Mantle cell lymphoma Antibody |
Zdroj: | Cytometry B Clin Cytom |
ISSN: | 1552-4949 |
Popis: | Background: Quantitative flow cytometry (QFCM) is being applied in the clinical flow cytometry laboratory for diagnosis, prognosis, and assessment of patients receiving antibody-based therapy. ABC values and the effect of technical variables on CD22 quantitation in acute lymphoblastic leukemia (ALL), chronic lymphocytic leukemia (CLL), mantle cell lymphoma (MCL), follicular lymphoma (FCL), hairy cell leukemia (HCL) and normal B cells were studied. Methods: The QuantiBrite System V R was used to determine the level of CD22 expression (mean antibody bound per cell, ABC) by malignant and normal B cells. The intra-assay variability, number of cells required for precision, effect of delayed processing as well as shipment of peripheral blood specimens (delayed processing and exposure to noncontrolled environments), and the effect of paraformaldehyde fixation on assay results were studied. Results: The QuantiBRITE method of measuring CD22 ABC is precise (median CV 1.6%, 95% confidence interval, 1.2–2.3%) but a threshold of 250 malignant cells is required for reliable CD22 ABC values. Delayed processing and overnight shipment of specimens resulted in significantly different ABC values whereas fixation for up to 12 h had no significant effect. ABC measurements determined that CD22 expression is lower than normal in ALL, CLL, FCL, and MCL but higher than normal in HCL. Conclusions: CD22 expression was atypical in the hematolymphoid malignancies studied and may have diagnostic utility. Technical variables such as cell number analyzed and delayed processing or overnight shipment of specimens impact significantly on the measurement of antigen expression by QFCM in the clinical laboratory. Published 2010 Wiley-Liss, Inc. † Key terms: CD22; quantitative flow cytometry; QuantiBRITE; ABC |
Databáze: | OpenAIRE |
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