Biophysical signatures of noncovalent aggregates formed by a glucagonlike peptide-1 analog: A prototypical example of biopharmaceutical aggregation
| Autor: | Mark L. Brader, Michael L. Roy, Allen H. Pekar, Brandon Doyle, Beth Ann Thomas, Mark J. Pollo |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Protein Folding
Circular dichroism Stereochemistry Biophysics Fluorescence spectrometry Pharmaceutical Science Peptide Biophysical Phenomena Protein Structure Secondary Fluorescence spectroscopy chemistry.chemical_compound Dynamic light scattering Glucagon-Like Peptide 1 Computational chemistry Spectroscopy Fourier Transform Infrared chemistry.chemical_classification Circular Dichroism Intermolecular force Dichroism Peptide Fragments Recombinant Proteins Freeze Drying Spectrometry Fluorescence Monomer Models Chemical Solubility chemistry Powders |
| Zdroj: | Journal of Pharmaceutical Sciences. 94:2749-2763 |
| ISSN: | 0022-3549 |
| DOI: | 10.1002/jps.20420 |
| Popis: | LY307161 is a 31 amino acid analog of glucagonlike peptide-1(7-37)OH susceptible to physical instability associated with pharmaceutical processing. Orthogonal biophysical studies were conducted to explore the origins of this physical instability and to distinguish pharmaceutically desirable states of this aggregating peptide from undesirable ones. Equilibrium sedimentation analysis established that LY307161 exists as a monomer at pH 3, and reversibly self-associates in the pH range 7.5–10.5. Causative factors for physical instability related to lyophilization conditions were investigated. Solution pH, acetonitrile content, and concentration of the peptide prior to lyophilization each impacted physicochemical properties of the resultant powders. A comparative study of two powder samples exhibiting physicochemically disparate properties established that LY307161 forms soluble noncovalent aggregates. FT-IR analyses in the solid and solution states identified a prominent band at 1657–1659 cm −1 attributed to α-helix structure. Noncovalent soluble aggregate exhibited characteristic bands at 1615 and 1698 cm −1 indicative of intermolecular β-sheet structure. An agitation-induced, precipitated solid form of LY307161 exhibited a different FT-IR signature indicative of a conformationally distinct species. Circular dichroism and fluorescence spectroscopy, together with dynamic light scattering measurements and dye-aggregate complexation, provided additional insights into the distinctions between aggregated and native LY307161. © 2005 Wiley-Liss, Inc. and the American Pharmacists Association. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text