Biological occupational allergy: Protein microarray for the study of laboratory animal allergy (LAA)
| Autor: | Danila Zennaro, Vittoria M Peri, Paola Melis, Maria Cristina Riviello, Stefania Massari, Annarita Wirz, Maria Concetta D’Ovidio, Chiara Rafaiani, Adriano Mari |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Allergy
Microarray occupational allergy laboratory animals protein microarray questionnaire health workers Immunoglobulin E Serum ige 03 medical and health sciences 0302 clinical medicine Medicine 030212 general & internal medicine Sensitization Asthma Allergy protein biology business.industry lcsh:Public aspects of medicine Laboratory animal allergy occupational allergy| laboratory animals| protein microarray| questionnaire| health workers lcsh:RA1-1270 General Medicine medicine.disease 030210 environmental & occupational health medicine.anatomical_structure Immunology biology.protein business Research Article |
| Zdroj: | AIMS Public Health, Vol 5, Iss 4, Pp 352-365 (2018) AIMS Public Health AIMS Public Health 5 (2018): 352–365. doi:10.3934/publichealth.2018.4.352 info:cnr-pdr/source/autori:Maria C D'Ovidio1,*, Annarita Wirz2, Danila Zennaro3, Stefania Massari4, Paola Melis1, Vittoria M Peri5, Chiara Rafaiani3, Maria C Riviello6 and Adriano Mari3/titolo:Biological occupational allergy: Protein microarray for the study of laboratory animal allergy (LAA)/doi:10.3934%2Fpublichealth.2018.4.352/rivista:AIMS Public Health/anno:2018/pagina_da:352/pagina_a:365/intervallo_pagine:352–365/volume:5 |
| ISSN: | 2327-8994 |
| DOI: | 10.3934/publichealth.2018.4.352/fulltext.html |
| Popis: | Background: Laboratory Animal Allergy (LAA) has been considered a risk for the workers since 1989 by the NIOSH. About one third of the Laboratory Animal Workers (LAWs) can manifest symptoms to LAA as asthma, rhinitis, conjunctivitis and cutaneous reactions. The prevalence of LAA-induced clinical symptoms has been estimated with a great variability (4–44%) also due to the different methodologies applied. Objective: Evaluate the prevalence of IgE positivity to mouse and rat allergens in LAWs and assess which factors are predisposing to sensitization among subjects exposed to laboratory animals in the workplace. Methods: One hundred LAWs were invited to fill out a questionnaire regarding current allergic symptoms, atopic history, home environment, previous and current occupational history. IgE reactivity versus specific allergens was evaluated with ImmunoCAP ISAC. Results: Out of one hundred LAWs, 18% had a serum susceptibility to mouse and/or rat allergens and 42% reported to have occupational allergy symptoms. Combining the results acquired by ImmunoCAP ISAC and questionnaire, 17% of LAWs have been defined as LAWs-LAA positive since they present a positive IgE response and allergy symptoms, 1% LAWs-LAA sensitized, 25% LAWs-LAA symptomatic and 57% LAWs-LAA negative. Presence of previous allergy symptoms in work and life environment were significantly related to LAWs-LAA positive/sensitized. Conclusions: The study aimed to define the immunological profile of LAWs using the proteomic array as an innovative approach in the study of environmental and occupational exposure to allergens. We suggested a definition of LAWs-LAA considering serum IgE response and presence of allergy symptoms. The proposed approach has the advantage to provide a standard methodology for evaluating the specific IgE responsiveness to animal allergens in specific workplace also considering the immunological profile of workers referred to exposure in life and occupational environment. |
| Databáze: | OpenAIRE |
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