Region-specific disruption of the blood-brain barrier following repeated inflammatory dural stimulation in a rat model of chronic trigeminal allodynia
| Autor: | Christina R. Maxwell, Michael L. Oshinsky, Nathan T. Fried, Melanie B. Elliott |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Migraine Disorders Stimulation Blood–brain barrier Article Capillary Permeability Rats Sprague-Dawley 03 medical and health sciences Trigeminal Caudal Nucleus 0302 clinical medicine Chronic Migraine medicine Animals Inflammation business.industry General Medicine Minocycline Trigeminal Neuralgia medicine.disease Rats Cortex (botany) Disease Models Animal 030104 developmental biology Allodynia medicine.anatomical_structure Migraine Blood-Brain Barrier Dura Mater Neurology (clinical) Inflammation Mediators medicine.symptom business 030217 neurology & neurosurgery medicine.drug Astrocyte |
| Zdroj: | Cephalalgia. 38:674-689 |
| ISSN: | 1468-2982 0333-1024 |
| Popis: | BackgroundThe blood-brain barrier (BBB) has been hypothesized to play a role in migraine since the late 1970s. Despite this, limited investigation of the BBB in migraine has been conducted. We used the inflammatory soup rat model of trigeminal allodynia, which closely mimics chronic migraine, to determine the impact of repeated dural inflammatory stimulation on BBB permeability.MethodsThe sodium fluorescein BBB permeability assay was used in multiple brain regions (trigeminal nucleus caudalis (TNC), periaqueductal grey, frontal cortex, sub-cortex, and cortex directly below the area of dural activation) during the episodic and chronic stages of repeated inflammatory dural stimulation. Glial activation was assessed in the TNC via GFAP and OX42 immunoreactivity. Minocycline was tested for its ability to prevent BBB disruption and trigeminal sensitivity.ResultsNo astrocyte or microglial activation was found during the episodic stage, but BBB permeability and trigeminal sensitivity were increased. Astrocyte and microglial activation, BBB permeability, and trigeminal sensitivity were increased during the chronic stage. These changes were only found in the TNC. Minocycline treatment prevented BBB permeability modulation and trigeminal sensitivity during the episodic and chronic stages.DiscussionModulation of BBB permeability occurs centrally within the TNC following repeated dural inflammatory stimulation and may play a role in migraine. |
| Databáze: | OpenAIRE |
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