High-Throughput Aminoacyl-tRNA Synthetase Engineering for Genetic Code Expansion in Yeast

Autor:	Jessica T. Stieglitz, James A. Van Deventer
Rok vydání:	2022
Předmět:	Amino Acyl-tRNA Synthetases RNA Transfer Genetic Code Biomedical Engineering Codon Terminator Escherichia coli General Medicine Saccharomyces cerevisiae Amino Acids Protein Engineering Biochemistry Genetics and Molecular Biology (miscellaneous)
Zdroj:	ACS synthetic biology. 11(7)
ISSN:	2161-5063
Popis:	Protein expression with genetically encoded noncanonical amino acids (ncAAs) benefits a broad range of applications, from the discovery of biological therapeutics to fundamental biological studies. A major factor limiting the use of ncAAs is the lack of orthogonal translation systems (OTSs) that support efficient genetic code expansion at repurposed stop codons. Aminoacyl-tRNA synthetases (aaRSs) have been extensively evolved in
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c453d550a0b7ee2181b8b32ff674153e https://pubmed.ncbi.nlm.nih.gov/35793554 Zobrazit plný text záznamu