Efficiency of the polymerase chain reaction amplification of the uid gene for detection of Escherichia coli in contaminated water
| Autor: | J. Robinson, Samina Iqbal, J.R. Saunders, Jonathan Porter, D. Deere, Clive Edwards |
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| Rok vydání: | 1997 |
| Předmět: |
DNA
Bacterial Recombinase Polymerase Amplification medicine.disease_cause Applied Microbiology and Biotechnology Polymerase Chain Reaction Microbiology law.invention chemistry.chemical_compound law Genes Regulator medicine Escherichia coli Gene Polymerase chain reaction Glucuronidase Bacteriological Techniques biology Sewage Multiple displacement amplification biology.organism_classification Enterobacteriaceae Molecular biology chemistry Evaluation Studies as Topic Genes Bacterial Water Microbiology Hot start PCR DNA |
| Zdroj: | Letters in applied microbiology. 24(6) |
| ISSN: | 0266-8254 |
| Popis: | Direct detection of Escherichia coli from polluted river water was achieved using polymerase chain reaction (PCR) amplification of the uid gene. Amplification using DNA from environmental samples resulted in non-specific DNA fragments. Specific amplification was achieved through use of the touch-down PCR procedure. Targeting the uidA structural region of the gene gave reproducibly better amplification than targeting the uidR regulatory region. The data demonstrate conditions for optimal specific detection. |
| Databáze: | OpenAIRE |
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