Phosphatidylinositol 4,5-bisphosphate regulation of SNARE function in membrane fusion mediated by CAPS
| Autor: | Judith A. Kowalchyk, Declan J. James, Chuenchanok Khodthong, Thomas Martin |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Phosphatidylinositol 4
5-Diphosphate Cancer Research Vesicle fusion Recombinant Fusion Proteins Biology Membrane Fusion PC12 Cells Article Exocytosis Vesicle tethering Phosphatidylinositol Phosphates Genetics Animals Molecular Biology SNARE complex assembly Qa-SNARE Proteins Calcium-Binding Proteins Cell Membrane SNAP25 Munc-18 Kiss-and-run fusion Rats Cell biology Liposomes Molecular Medicine biological phenomena cell phenomena and immunity SNARE Proteins SNARE complex Protein Binding |
| Zdroj: | Advances in Enzyme Regulation. 50:62-70 |
| ISSN: | 0065-2571 |
| DOI: | 10.1016/j.advenzreg.2009.10.012 |
| Popis: | Ca2+-triggered vesicle exocytosis in neuro endocrine cells requires priming reactions that follow vesicle tethering/docking and precede triggered fusion. Priming requires PI(4,5)P2 and priming factors, and likely involves SNARE protein complex assembly. In studies with proteoliposomes, the priming factor CAPS interacts with PI(4,5)P2, binds the SNARE protein syntaxin-1, promotes trans SNARE complex formation, and stimulates PI(4,5)P2- and SNARE-dependent liposome fusion. We propose that CAPS functions in priming vesicle exocytosis by coupling membrane binding to SNARE complex assembly. |
| Databáze: | OpenAIRE |
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