Exercise-mimicking treatment fails to increase Fndc5 mRNAirisin secretion in primary human myotubes
| Autor: | Vitazoslav Belan, Timea Kurdiova, Christian Wolfrum, Barbara Ukropcova, Alexander Mayer, Miroslav Balaz, Denisa Maderova, Jozef Ukropec |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Physiology Muscle Fibers Skeletal 030209 endocrinology & metabolism White adipose tissue Biology Biochemistry Prediabetic State 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Endocrinology Internal medicine Myokine medicine Myocyte Humans Obesity RNA Messenger Muscle Skeletal Exercise Cells Cultured 030304 developmental biology 0303 health sciences Forskolin Myogenesis Ionomycin Colforsin Skeletal muscle FNDC5 Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Fibronectins medicine.anatomical_structure chemistry Diabetes Mellitus Type 2 Transcription Factors |
| Zdroj: | Peptides |
| ISSN: | 1873-5169 |
| Popis: | Irisin myokine secreted by skeletal muscle was suggested to mediate some of exercise health benefits via "browning" of white adipose tissue. However mounting evidence contradicts the regulatory role of exercise for muscle irisin production/secretion in humans. Thus we explored the direct effect of exercise mimicking treatment on irisin in human primary muscle cells in vitro. Human primary muscle cell cultures were established from lean obese prediabetic and type 2 diabetic individuals. Complex metabolic phenotyping included assessment of insulin sensitivity (euglycemic hyperinsulinemic clamp) and adiposity contentdistribution (MRIMRS). In vitro exercise mimicking treatment (forskolin + ionomycin) was delivered in 1 h pulse/day during differentiation. Fndc5 mRNA (qRT PCR) and secreted irisin (ELISA) were determined in cells and media. Exercise mimicking treatment more than doubled Pgc1a mRNA in differentiated muscle cells. Nevertheless Fndc5 mRNA was reduced by 18 and irisin in media by 20. Moreover Fncd5 mRNA was increased in myotubes derived from individuals with type 2 diabetes independent on exercise mimicking treatment. Fndc5 mRNA in cells was positively related to fasting glycemia (p = 0.0001) and negatively to whole body insulin sensitivity (p < 0.05). Collectively our data do not support the role of exercise related signaling pathways in irisin regulation in human skeletal muscle and confirm our previous observations on increased Fndc5 expression in muscle cells from individuals with type 2 diabetes. © 2014 Published by Elsevier Inc. |
| Databáze: | OpenAIRE |
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