Study of the experimental conditions for the lipase production by a newly isolated strain of Pseudomonas aeruginosa for the enantioselective hydrolysis of (±)-methyl trans-3(4-methoxyphenyl) glycidate
| Autor: | Rakesh K. Jain, Gurmeet Kaur, Asit K. Chakraborti, Sawraj Singh, Uttam Chand Banerjee |
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| Rok vydání: | 2006 |
| Předmět: |
Bioengineering
medicine.disease_cause Hydrolysis Isomerism Species Specificity medicine Organic chemistry Inducer Lipase Enantiomeric excess Chromatography Strain (chemistry) biology Pseudomonas aeruginosa General Medicine biology.organism_classification Enzyme Activation Yield (chemistry) biology.protein Epoxy Compounds Propionates Bacteria Biotechnology |
| Zdroj: | Bioprocess and Biosystems Engineering. 28:341-348 |
| ISSN: | 1615-7605 1615-7591 |
| DOI: | 10.1007/s00449-005-0039-7 |
| Popis: | A lipase producing bacterium has been isolated from the soil to enantiospecifically hydrolyze the (+/-)-methyl trans-3(4-methoxyphenyl) glycidate (MPGM), an intermediate in the synthesis of cardiovascular drug, diltiazem. This hydrolysis provided the desired (-)-MPGM in 44% yield with 99% enantiomeric excess. The organism was identified and confirmed as Pseudomonas aeruginosa by 16S rRNA sequencing. The various physiochemical parameters have been optimized for the maximum production of lipase in shake flask. Beef extract was found to be the best nitrogen source for lipase production. The optimized cultivation conditions were 30 degrees C with an initial medium pH 8 in shake flask. Both inoculum age and inoculum concentration have positive effect on the lipase production and (+/-)-MPGM (3 mM) was found to be the optimal inducer. |
| Databáze: | OpenAIRE |
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