Determination of the Rate-Limiting Step in Fatty Acid Transport
| Autor: | John C. Conboy, Victoria Cheng, Dylan R. Kimball |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
010304 chemical physics Chemistry Fatty Acids Lipid Bilayers Fatty acid Biological Transport Chromosomal translocation 010402 general chemistry Rate-determining step 01 natural sciences 0104 chemical sciences Surfaces Coatings and Films Kinetics Adsorption Membrane 0103 physical sciences Phosphatidylcholines Materials Chemistry Biophysics Thermodynamics Physical and Theoretical Chemistry Stearic Acids |
| Zdroj: | The Journal of Physical Chemistry B. 123:7157-7168 |
| ISSN: | 1520-5207 1520-6106 |
| DOI: | 10.1021/acs.jpcb.9b05162 |
| Popis: | The generally accepted model of free fatty acid (FA) transport through cellular membranes occurs in three steps, adsorption of the FA onto the membrane, translocation across the membrane ("flip-flop"), and subsequent desorption of the FA into the cytosol. There still exists some dispute as to the identity of the rate-limiting step of FA transport. In the present study, sum-frequency vibrational spectroscopy (SFVS) was used to directly measure the rate of stearic acid (SA) flip-flop in planar supported lipid bilayers (PSLBs) comprised of 1,2-distearoyl |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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