Transcriptome analysis of hepatopancreas of Eriocheir sinensis with hepatopancreatic necrosis disease (HPND)

Autor: Lei Xiaoqing, Jiang Qinglong, Kun Hu, Xiong Chunxian, Zongying Yang, Xu Liangqing, Yao Yi, Zeng Liugen, Yulan Wang, Hou Yujie, Yan Baohua
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Enzyme Metabolism
Hepatopancreas
Gene Expression
Crabs
Biochemistry
Transcriptome
Cytochrome P-450 Enzyme System
Drug Metabolism
Medicine and Health Sciences
Enzyme Chemistry
Genetics
Chinese mitten crab
Multidisciplinary
biology
Gene Ontologies
Eukaryota
04 agricultural and veterinary sciences
Genomics
Crustaceans
Eriocheir
Organelle part
Medicine
Metabolic Pathways
Biological regulation
Transcriptome Analysis
Research Article
Arthropoda
Brachyura
Science
Xenobiotics
03 medical and health sciences
Necrosis
Animals
Xenobiotic Metabolism
Pharmacokinetics
KEGG
Gene
Pharmacology
Organisms
Biology and Life Sciences
Computational Biology
biology.organism_classification
Genome Analysis
Invertebrates
030104 developmental biology
Metabolism
040102 fisheries
Enzymology
0401 agriculture
forestry
and fisheries
Zdroj: PLoS ONE, Vol 15, Iss 2, p e0228623 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Hepatopancreatic necrosis disease (HPND) is a newly emerging disease in the Chinese mitten crab, Eriocheir sinensis, which has resulted in large economic losses. However, the underlying cause of this disease remains unclear. To better understand the pathogenesis and pathogenic mechanism of HPND, we compared the transcriptome differences of the hepatopancreas of E. sinensis with and without HPND. The analysis yielded > 30 million reads for each sample of three test (with HPND) and three control groups (without HPND). We observed 978 downregulated genes and 644 upregulated genes. Among the gene ontology categories "biological process," "cellular component," and "molecular function", the subcategories cellular process, single-organism process, biological regulation, metabolic process, cell part, organelle, organelle part, binding, and catalytic were enriched. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that "metabolism of xenobiotics by cytochrome P450," "drug metabolism-cytochrome P450," "chemical carcinogenesis," and "material metabolism" were the "five" most significantly enriched pathways in the hepatopancreas of E. sinensis with HPND. The results revealed that material metabolic abnormalities and drug effects from the external environment might be associated with HPND in the Chinese mitten crab. Considering the wide use of pyrethroids for pond cleaning in Xinghua city, we speculated that pyrethroids might cause HPND in the Chinese mitten crab. Our study provided useful information about the cause and pathogenetic mechanisms of HPND and could help to prevent this disease in production practice.
Databáze: OpenAIRE
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