Influence of different fluid resuscitation techniques on the number of myeloid-derived suppressor cells in rats
| Autor: | Dou Hh, Zhenjie Wang, Lei Li, Liang Wang, Hui Wang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Resuscitation
Ringer's Lactate medicine.medical_treatment Flow cytometry Sham group Andrology Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Immune system Genetics Medicine Animals Molecular Biology Saline Solution Hypertonic medicine.diagnostic_test business.industry Myeloid-Derived Suppressor Cells 030208 emergency & critical care medicine Immunosuppression General Medicine Crystalloid Solutions Hypertonic saline Rats 030220 oncology & carcinogenesis Rehydration Solutions Hemorrhagic shock Myeloid-derived Suppressor Cell Isotonic Solutions business Spleen |
| Zdroj: | Genetics and molecular research : GMR. 15(2) |
| ISSN: | 1676-5680 |
| Popis: | We investigated the influence of different fluid resuscitation techniques on the number of myeloid-derived suppressor cells (MDSCs) in rats. Seventy-two healthy Sprague-Dawley rats were randomly divided into groups that received sham operation (Sham group), hypertonic saline (HRS group), lactated ringer's solution (LRS group), or crystalloid solution (LCRS group). Six rats from each group were sacrificed by cervical dislocation at 12, 24, and 48 h after resuscitation. The spleens were harvested under sterile conditions and spleen cell suspension was prepared. The number of MDSCs was detected using flow cytometry. The number of MDSCs in the Sham group did not differ significantly among the different time points. Compared with the Sham group, the number of MDSCs after the use of the different fluid resuscitation techniques increased to varying extents and the differences among the groups were significant. The number of MDSCs in the HRS group was much lower than that of the LRS and LCRS groups at both 24 and 48 h (P < 0.05). At 12 h, the number of MDSCs in the HRS group was significantly lower than that of the LRS group (P < 0.05). The differences between the HRS and LCRS groups were not statistically significant. Shortly after hemorrhagic shock resuscitation, the immune function of rats was suppressed to a varying extent and was gradually restored over time. Resuscitation with HRS alleviated the immunosuppression at the early stage after shock. |
| Databáze: | OpenAIRE |
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