Expansion of CD8+ cells in autoimmune hemolytic anemia

Autor:	S Ju Smirnova, Sergei M. Kulikov, Ju V Sidorova, Tsvetaeva Nv, O. F. Nikulina, B V Biderman, Elena E. Nikulina, Andrey Sudarikov
Rok vydání:	2016
Předmět:	Adult Erythrocyte Indices Male 0301 basic medicine Adolescent medicine.medical_treatment Immunology Splenectomy Disease CD8-Positive T-Lymphocytes Biology Gene Rearrangement T-Lymphocyte Immunophenotyping Young Adult 03 medical and health sciences Autoimmune Process T-Lymphocyte Subsets medicine Humans Immunology and Allergy Lymphocyte Count Gene Rearrangement B-Lymphocyte Aged Base Sequence Sequence Analysis DNA Middle Aged medicine.disease Hemolysis Phenotype 030104 developmental biology Antigens Surface Monoclonal Female Anemia Hemolytic Autoimmune Autoimmune hemolytic anemia CD8
Zdroj:	Autoimmunity. 49:147-154
ISSN:	1607-842X 0891-6934
Popis:	Autoimmune hemolytic anemia (AIHA) is a rare blood disease associated with the production of auto-antibodies and autoimmune hemolysis. A critical role of B-cells in the development of AIHA has been demonstrated before. Here, we present the analysis of the clonal T-cell populations in patients with AIHA. Thirty-three patients with AIHA were included in this study. Thirteen patients with other anemias, 14 patients with other autoimmune conditions (SLE - 6, RA - 8) and 20 healthy donors were included in the study as a control group. The clonality of T-cell was evaluated by the assessment of the T-cell receptor gamma and beta chain gene rearrangements (TCRG and TCRB). The incidence of T-cell monoclonality detected in patients with AIHA was significantly higher compared to the control group. The persistence of T-cell clones did not correlate with the level of hemoglobin and other signs of remission or relapse and did not disappear after the therapy and clinical improvement (observation period was between 1 and 10 years). There was no correlation between the T-cell clonality and the gender, age, splenectomy, duration or severity of the disease. Fractionation of T-lymphocytes (CD4+, CD8+, CD4+25+) revealed that the monoclonal T-cells belonged to the CD8+ sub-population. We assume that besides a possible causative role of the T-cell clones in AIHA to autoimmune process, these clones do not directly participate in the development and maintenance of hemolysis. Most of the AIHA patients (48.5%) demonstrated a T-cell monoclonality, which requires monitoring and should be distinguished from T-cell tumors.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c43b6790c0e1648f28833344b16b2542 https://doi.org/10.3109/08916934.2016.1138219 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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