Intracellular Distribution of Manganese by the Trans-Golgi Network Transporter NRAMP2 Is Critical for Photosynthesis and Cellular Redox Homeostasis
| Autor: | Rémy Cailliatte, Frédéric Domergue, Santiago Alejandro, Catherine Curie, David Correia, Carine Alcon, Léon Dirick, Loren Castaings, Jean-François Briat, Stéphane Mari |
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| Přispěvatelé: | Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux, Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine [SDV]Life Sciences [q-bio] Friends Plant Science Vacuole medicine.disease_cause 01 natural sciences Superoxide dismutase 03 medical and health sciences symbols.namesake photosystème medicine oxidative stress [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Homeostasis Photosynthesis ComputingMilieux_MISCELLANEOUS Research Articles chemistry.chemical_classification Reactive oxygen species transporteur de metaux Manganese biology arabidopsis thaliana stress oxydatif Cell Biology Golgi apparatus Cell biology Chloroplast Cytosol 030104 developmental biology manganèse chemistry Biochemistry biology.protein symbols [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Oxidation-Reduction Oxidative stress Intracellular 010606 plant biology & botany trans-Golgi Network |
| Zdroj: | The Plant cell The Plant cell, American Society of Plant Biologists (ASPB), 2017, 29 (12), pp.3068-3084. ⟨10.1105/tpc.17.00578⟩ The Plant cell, American Society of Plant Biologists (ASPB), 2018, 29 (12), pp.3068-3084. ⟨10.1105/tpc.17.00578⟩ |
| ISSN: | 1040-4651 1532-298X |
| Popis: | Plants require trace levels of manganese (Mn) for survival, as it is an essential cofactor in oxygen metabolism, especially O2 production via photosynthesis and the disposal of superoxide radicals. These processes occur in specialized organelles, requiring membrane-bound intracellular transporters to partition Mn between cell compartments. We identified an Arabidopsis thaliana member of the NRAMP family of divalent metal transporters, NRAMP2, which functions in the intracellular distribution of Mn. Two knockdown alleles of NRAMP2 showed decreased activity of photosystem II and increased oxidative stress under Mn-deficient conditions, yet total Mn content remained unchanged. At the subcellular level, these phenotypes were associated with a loss of Mn content in vacuoles and chloroplasts. NRAMP2 was able to rescue the mitochondrial yeast mutant mtm1∆ In plants, NRAMP2 is a resident protein of the trans-Golgi network. NRAMP2 may act indirectly on downstream organelles by building up a cytosolic pool that is used to feed target compartments. Moreover, not only does the nramp2 mutant accumulate superoxide ions, but NRAMP2 can functionally replace cytosolic superoxide dismutase in yeast, indicating that the pool of Mn displaced by NRAMP2 is required for the detoxification of reactive oxygen species. |
| Databáze: | OpenAIRE |
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