Screening of 214 single nucleotide polymorphisms in 44 candidate cancer susceptibility genes: a case-control study on gastric and colorectal cancers in the Japanese population
| Autor: | Motoki Iwasaki, Yoshio Kasuga, Syusuke Natsukawa, Shoichiro Tsugane, Sumiko Ohnami, Shinobu Ikeda, Hiromi Sakamoto, Shizuka Sasazuki, Yoichi Koizumi, Teruhiko Yoshida, Kozo Shaura |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male medicine.medical_specialty Colorectal cancer Single-nucleotide polymorphism Gastroenterology Polymorphism Single Nucleotide Cohort Studies Asian People Japan Polymorphism (computer science) Stomach Neoplasms Internal medicine medicine Humans Genetic Predisposition to Disease Genetic Testing Stomach cancer Gene Life Style Genetic testing Aged Hepatology medicine.diagnostic_test business.industry Case-control study Cancer Middle Aged medicine.disease Case-Control Studies Cancer research Female business Colorectal Neoplasms |
| Zdroj: | The American journal of gastroenterology. 103(6) |
| ISSN: | 1572-0241 |
| Popis: | Single nucleotide polymorphisms (SNPs) have potential as markers for identifying genes responsible for common diseases and for personalized medicine. To investigate the association between polymorphisms and gastrointestinal (gastric and colorectal) cancer, we performed a hospital-based case-control study in Japan.We screened a total of 214 SNPs in 44 candidate genes by using a mass spectrometry-based technique (MassARRAY; Sequenom, Inc., San Diego, CA). In this study, 153 patients and 302 controls for gastric cancer and 121 patients and 245 controls for colorectal cancer were matched with regard to age, sex, and residential area. Genes were selected based on their possible interactions with the environment and lifestyle, and the candidate genes constitute those encoding xenobiotic metabolism enzymes, DNA repair enzymes, and other stress-related proteins. Each polymorphism was tested in controls to ensure its fit with Hardy-Weinberg equilibrium. Odds ratios (OR) and 95% confidence intervals (CI) were calculated by conditional logistic regression analysis to estimate the association between genetic polymorphisms and the risk of gastric and colorectal cancers.Twenty-one SNPs in nine genes were associated with the risk of gastric cancer (P0.05) and 15 SNPs in nine genes were associated with the risk of colorectal cancer (P0.05).The findings of the present study will be the basis for future large-scale association studies of gene-environment factors using the candidate gene approach for the Japanese population. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|