Polyelectrolyte Multilayer-Mediated Gene Delivery for Semaphorin Signaling Pathway Control
| Autor: | Joelle Ogier, Florent Meyer, Jean-Claude Voegel, Doriane Richard, Isabelle Nguyen, Dominique Bagnard, Christine Affolter, Pierre Schaaf |
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| Rok vydání: | 2010 |
| Předmět: |
Cell signaling
Polymers Blotting Western Cell Nerve Tissue Proteins Semaphorins Biology Gene delivery Transfection Biomaterials Plasmid Semaphorin Chlorocebus aethiops Neuropilin 1 medicine Animals General Materials Science Gene Transfer Techniques SEMA3A General Chemistry Immunohistochemistry Molecular biology Neuropilin-1 Cell biology medicine.anatomical_structure COS Cells Sulfonic Acids Signal transduction Signal Transduction Biotechnology |
| Zdroj: | Small. 6:2405-2411 |
| ISSN: | 1613-6810 |
| DOI: | 10.1002/smll.201000228 |
| Popis: | The capability of multilayered polyelectrolyte films (MPFs) to control the sequential expression of two genes encoding cell receptors involved in a common cell signalling activity is shown, while achieving a fully functional signal transduction. As a functional model system representative of a cell signalling process that proceeds in a top-down manner, cell collapse induced by semaphorin 3A (Sema3A) was chosen as the target. Polyelectrolyte multilayers were sequentially functionalized with two plasmids encoding Neuropilin-1 (NRP-1) and Plexin-A1 (Plx-A1), respectively, acting as co-receptors for Sema3A. By using hyaluronan and chitosan as structural components for the incorporation of plasmid DNA layers onto precursor films made of poly-allylamine hydrochloride and poly-sodium-4-styrenesulfonate, the polyelectrolyte system is established; this systems is capable of delivering both plasmids to Cos-1 cells in a manner that permits control over the timing and the respective order in which the two plasmid DNA constructs are expressed. Importantly, it was observed that, following Sema3A stimulation, COS-1 cells co-expressing Plx-A1 and NRP-1 display a collapse phenotype, which is determined by the multilayer build-up scheme, and that the expression products of both transgenes embedded in MPFs are temporally functional over several days while acting their role of co-receptors for Sema3A. |
| Databáze: | OpenAIRE |
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