Testicular Isoform of Angiotensin I-Converting Enzyme (ACE, CD143) on the Surface of Human Spermatozoa: Revelation and Quantification Using Monoclonal Antibodies
| Autor: | Kulakov Vi, Marina A. Alexinskaya, M. A. Nikolaeva, Folker E. Franke, Roman Metzger, Ronald F. Albrecht, Gennady T. Sukhikh, Irina V. Balyasnikova, Sergei M. Danilov |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Gene isoform endocrine system medicine.drug_class Immunology Immunocytochemistry CHO Cells Peptidyl-Dipeptidase A Biology Monoclonal antibody Flow cytometry Cricetinae medicine Animals Humans Immunology and Allergy Distribution (pharmacology) Ejaculation Cells Cultured chemistry.chemical_classification medicine.diagnostic_test urogenital system Cell Membrane Antibodies Monoclonal Obstetrics and Gynecology Flow Cytometry Angiotensin I converting enzyme Immunohistochemistry Spermatozoa Sperm Molecular biology Enzyme Reproductive Medicine chemistry Sperm Motility |
| Zdroj: | American Journal of Reproductive Immunology. 55:54-68 |
| ISSN: | 1600-0897 8755-8920 |
| DOI: | 10.1111/j.1600-0897.2005.00326.x |
| Popis: | Problem The elucidation of the role of angiotensin-converting enzyme (ACE, CD143) in the male fertility has been hampered by the absence of highly specific antibodies to the native testicular isoform (tACE). The quantification of tACE expression on human-ejaculated spermatozoa was performed using a novel panel of monoclonal antibodies (mAbs). Method of study The expression of tACE on the surface of live and fixed human spermatozoa was analyzed by flow cytometry and immunocytochemistry using new mAbs to human tACE. Results Monoclonal antibodies 1E10 and 4E3 similarly revealed tACE on the surface of live and fixed spermatozoa. The high percentage of tACE-positive spermatozoa (median 81%) was revealed in the swim-up fraction of sperm. Antibody-induced tACE shedding occurs preferentially from live sperm with defective function and/or morphology. Testicular ACE is located on the plasma membrane of the post-acrosomal region, the neck and midpiece of normal spermatozoa, but showed a variable distribution on the defective cells. Conclusions The new mAbs recognizing the C-terminal domain of human ACE are useful tools for quantification of tACE expression on human live and fixed spermatozoa and further adequate analysis of the tACE role in reproduction. |
| Databáze: | OpenAIRE |
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