Arboviruses isolated from mosquitoes collected from urban and peri-urban areas of eastern Australia
| Autor: | Bruce Harrower, Ian L. Pritchard, Roy A. Hall, Alyssa T. Pyke, Andrew F. van den Hurk, Richard C. Russell, Cassie C. Jansen, Cameron E. Webb, Paul Zborowski, Scott A. Ritchie, Natalie A. Prow |
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| Přispěvatelé: | Jansen, Cassie C, Prow, Natalie A, Webb, Cameron E, Hall, Roy A, Pyke, Alyssa T, Harrower, Bruce J, Pritchard, Ian L, Zborowski, Paul, Ritchie, Scott A, Russell, Richard C, van den Hurk, Andrew F |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Veterinary medicine
viruses detection mosquito Alphavirus Arbovirus Virus Ross River virus parasitic diseases medicine Animals Cities Ecology Evolution Behavior and Systematics Ecosystem Demography Aedes biology Public Health Environmental and Occupational Health Australia General Medicine biology.organism_classification medicine.disease Virology Flavivirus Culicidae Insect Science Vector (epidemiology) Population Surveillance Barmah Forest virus urban Arboviruses Environmental Monitoring |
| Popis: | To determine the presence of arboviruses in mosquito populations from major urban areas of eastern Australia, a total of 67,825 mosquitoes, representing ∼60 species, was collected and tested from Cairns, Brisbane, and Sydney between January 2005 and April 2008. Mosquito pools were screened by inoculation onto mosquito cell cultures and the detection of viral antigen using a panel of flavivirus and alphavirus monoclonal antibodies in an enzyme-linked immunosorbent assay. Suspect positive samples were confirmed using virus-specific real-time reverse transcriptasepolymerase chain reaction assays. No flaviviruses were detected, but 2 alphaviruses were isolated from mosquito pools collected from Cairns, with 1 Barmah Forest virus isolate from a pool of 100 Aedes vigilax and 1 Ross River virus isolate from a pool of 83 Verrallina carmenti. In addition, a single Aedes alternans collected from Sydney yielded an isolate most similar to Stretch Lagoon virus, a newly described virus from the genus Orbivirus. These results suggest that during the study, arboviruses were circulating at a low level in the areas sampled. The findings from this study will promote public health awareness of the risk of arboviruses in urban areas, leading to more informative public health campaigns to safeguard the Australian public. Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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