A rapid, two-step method for high-yield purification of recombinant rat acidic and basic fibroblast growth factors
| Autor: | Andrew G. Karaganis, Sydney A. Shain, Li Dao Ke |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Isopropyl Thiogalactoside
Molecular Sequence Data Cell Fractionation medicine.disease_cause Fibroblast growth factor Chromatography Affinity law.invention law Escherichia coli medicine Animals Amino Acid Sequence Amino Acids Chromatography High Pressure Liquid Polymerase Chromatography Base Sequence Dose-Response Relationship Drug biology Heparin Chemistry Sepharose Biological activity Fibroblasts beta-Galactosidase Recombinant Proteins Rats Membrane medicine.anatomical_structure Biochemistry Enzyme Induction biology.protein Recombinant DNA Fibroblast Growth Factor 1 Biological Assay Fibroblast Growth Factor 2 Cell Division Biotechnology |
| Zdroj: | Protein Expression and Purification. 3:497-507 |
| ISSN: | 1046-5928 |
| DOI: | 10.1016/1046-5928(92)90067-7 |
| Popis: | We describe the preparation of vectors for T7 polymerase-driven, high-level expression of rat acidic (aFGF) or basic (bFGF) fibroblast growth factors in Escherichia coli . Following isopropyl-β- d -thiogalacto-side induction of T7 polymerase, rat aFGF or bFGF represented a major portion of the proteins synthesized by vector-transformed E. coli . Passage of cell extracts through an Amicon YM-100 membrane provided ultrafiltrates containing either aFGF or bFGF as the principal component. A single heparin-Sepharose chromatography of the ultrafiltrates yielded essentially homogenous, biologically active, recombinant rat aFGF or bFGF. By silanizing vessels and using buffers containing 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid, we could store homogenous aFGF or bFGF preparations without significant loss during repeated freezing and thawing. Previous methods for purifying aFGF or bFGF utilized salt fractionation followed by sequential ion exchange and heparin-Sepharose chromatography. These purified aFGF or bFGF preparations routinely were stored in buffered carrier protein to minimize mitogen loss resulting from adsorption to glass or plastic surfaces. In contrast, the methods that we detail are rapid, require minimal manipulation of preparations, and permit storage of carrier-free, homogenous preparations without loss resulting from surface adsorption. The protocols can be used for preparation of homogenous aFGF or bFGF of other species and may be readily applied to the isolation and characterization of FGF-like mitogens present in cultured cell extracts, conditioned medium, or tissue preparations. |
| Databáze: | OpenAIRE |
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