Effect of ethidium bromide intercalation on DNA radiosensitivity
| Autor: | V. Michalik, Melanie Spotheim-Maurizot, Michel Charlier, M. Begusova |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Radiation-Protective Agents
Biology Radiation Tolerance Fast Neutrons chemistry.chemical_compound Plasmid Ethidium Escherichia coli Radiology Nuclear Medicine and imaging Nucleotide Gel electrophoresis chemistry.chemical_classification Electrophoresis Agar Gel Radiological and Ultrasound Technology DNA Superhelical Hydroxyl Radical Nucleic acid sequence DNA Free Radical Scavengers Molecular biology PBR322 Intercalating Agents chemistry Agarose gel electrophoresis Nucleic Acid Conformation Ethidium bromide DNA Damage Plasmids |
| Zdroj: | International journal of radiation biology. 76(1) |
| ISSN: | 0955-3002 |
| Popis: | To assess the influence of the intercalating drug ethidium bromide (EtBr) on the yields of single strand breaks (ssb) induced by fast neutrons in supercoiled pBR322 plasmid and in a linear DNA restriction fragment.The yield of ssb in the plasmid was measured by agarose gel electrophoresis. The proportion of fragments bearing one ssb and the probability of breakage at each nucleotide site was determined using sequencing gel electrophoresis. The volume variations due to the intercalation of EtBr were calculated. The expected radio-modifying effect at each nucleotide site of the linear fragment was evaluated using a reported simulation procedure.The ssb yield in the plasmid increased for concentrations up to 0.04 drug/bp and fell back in the range 0.04-0.1 drug/bp. For the linear DNA, only a slight protective effect was observed over the whole concentration range. The effect was almost the same at all nucleotide sites.For the linear DNA fragment, radioprotection was mainly due to scavenging of OH* radicals by the intercalated drug. For the plasmid, the radio-modifying effect results mainly from the variation of its effective volume, due to the modification of superhelicity. |
| Databáze: | OpenAIRE |
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