Two types of transgenic lines for doxycycline-inducible, cell-specific gene expression in zebrafish ultraviolet cone photoreceptors
| Autor: | Abbie M. Jensen, Leah J. Campbell, Megan C. West, John J. Willoughby |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
animal structures
Transgene Genetic Vectors Fluorescent Antibody Technique Biology Response Elements Article Cell Line Green fluorescent protein Animals Genetically Modified Transactivation Gene Order Gene expression Genetics Animals Molecular Biology Gene Zebrafish Regulation of gene expression biology.organism_classification Molecular biology Gene Expression Regulation Organ Specificity Cell culture Doxycycline Retinal Cone Photoreceptor Cells Trans-Activators sense organs Developmental Biology |
| Zdroj: | Gene Expression Patterns. 14:96-104 |
| ISSN: | 1567-133X |
| Popis: | Temporal and spatial control of gene expression is important for studying the molecular and cellular mechanisms of development, physiology, and disease. We used the doxycycline (Dox)-inducible, Tet-On system to develop transgenic zebrafish for inducible, cell specific control of gene expression in the ultraviolet (UV) cone photoreceptors. Two constructs containing the reverse tetracycline-controlled transcriptional transactivator (rtTA) gene driven by the UV opsin-specific promoter (opn1sw1) were used to generate stable transgenic zebrafish lines using the Tol2-based transgenesis method. One construct included a self-reporting GFP (opn1sw1:rtTA, TRE:GFP) and the other incorporated an epitope tag on the rtTA protein (opn1sw1:rtTA(flag)). UV cone-specific expression of TRE-controlled transgenes was induced by Dox treatment in larvae and adults. Induction of gene expression was observed in 96% of all larval UV cones within 16 h of Dox treatment. UV cone-specific expression of two genes from a bidirectional TRE construct injected into one-cell Tg(opn1sw1:rtTA(flag)) embryos were also induced by Dox treatment. In addition, UV cone-specific expression of Crb2a(IntraWT) was induced by Dox treatment in progeny from crosses of the TRE-response transgenic line, Tg(TRE:HA-Crb2a(IntraWT)), to the Tg(opn1sw1:rtTA, TRE:GFP) line and the Tg(opn1sw1:rtTA(flag)) line. These lines can be used in addition to the inducible, rod-specific gene expression system from the Tet-On Toolkit to elucidate the photoreceptor-specific effects of genes of interest in photoreceptor cell biology and retinal disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect