Aptasensor for the Detection of Mycobacterium tuberculosis in Sputum Utilising CFP10-ESAT6 Protein as a Selective Biomarker
| Autor: | Nor Azah Yusof, Jaafar Abdullah, Fatin Nabilah Mohd Faudzi, Faruq Mohammad, Umi Zulaikha Mohd Azmi, Siti Suraiya, Hamad A. Al-Lohedan, Shahrul Ainliah Alang Ahmad, Nurul Hanun Ahmad Raston, Sachin K. Khiste |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Working electrode
iron/gold magnetic nanoparticles General Chemical Engineering Aptamer portable detection systems differential pulse voltammetry (DPV) Mycobacterium tuberculosis chemistry.chemical_compound medicine General Materials Science Bovine serum albumin QD1-999 Detection limit CFP10-ESAT6 Chromatography biology Chemistry biology.organism_classification electrochemical aptasensor biology.protein Sputum Glutaraldehyde medicine.symptom Biosensor |
| Zdroj: | Nanomaterials, Vol 11, Iss 2446, p 2446 (2021) Nanomaterials Volume 11 Issue 9 |
| ISSN: | 2079-4991 |
| Popis: | A portable electrochemical aptamer-antibody based sandwich biosensor has been designed and successfully developed using an aptamer bioreceptor immobilized onto a screen-printed electrode surface for Mycobacterium tuberculosis (M. tuberculosis) detection in clinical sputum samples. In the sensing strategy, a CFP10-ESAT6 binding aptamer was immobilized onto a graphene/polyaniline (GP/PANI)-modified gold working electrode by covalent binding via glutaraldehyde linkage. Upon interaction with the CFP10-ESAT6 antigen target, the aptamer will capture the target where the nano-labelled Fe3O4/Au MNPs conjugated antibody is used to complete the sandwich format and enhance the signal produced from the aptamer–antigen interaction. Using this strategy, the detection of CFP10-ESAT6 antigen was conducted in the concentration range of 5 to 500 ng/mL. From the analysis, the detection limit was found to be 1.5 ng/mL, thereby demonstrating the efficiency of the aptamer as a bioreceptor. The specificity study was carried out using bovine serum albumin (BSA), MPT64, and human serum, and the result demonstrated good specificity that is 7% higher than the antibody–antigen interaction reported in a previous study. The fabricated aptasensor for M. tuberculosis analysis shows good reproducibility with an relative standard deviation (RSD) of 2.5%. Further analysis of M. tuberculosis in sputum samples have shown good correlation with the culture method with 100% specificity and sensitivity, thus making the aptasensor a promising candidate for M. tuberculosis detection considering its high specificity and sensitivity with clinical samples. |
| Databáze: | OpenAIRE |
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