Magnetic resonance spectroscopy reveals an impaired brain metabolic profile in mice resistant to cerebral malaria infected with Plasmodium berghei ANKA
Autor: | Angèle Viola, Jean-Marc Gulian, Marie-France Penet, Agnès Liprandi, Georges E. Grau, Nicolas Coltel, Yann Le Fur, Patrick Pj Cozzone, Frank Kober, Christiane Dalmasso, Sylviane Confort-Gouny |
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Přispěvatelé: | Centre de résonance magnétique biologique et médicale (CRMBM), Assistance Publique - Hôpitaux de Marseille (APHM)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS) |
Rok vydání: | 2007 |
Předmět: |
[SPI.OTHER]Engineering Sciences [physics]/Other
Magnetic Resonance Spectroscopy [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging Anemia Plasmodium berghei Malaria Cerebral Brain Edema Biology Biochemistry Brain Ischemia 03 medical and health sciences Mice 0302 clinical medicine parasitic diseases medicine Animals Molecular Biology Hepatic encephalopathy 030304 developmental biology Cell Proliferation 0303 health sciences Brain Diseases Mice Inbred BALB C Alanine Brain Cell Biology Jaundice medicine.disease biology.organism_classification 3. Good health Glutamine Cerebral blood flow Liver Cerebral Malaria Cerebrovascular Circulation Immunology Female medicine.symptom 030217 neurology & neurosurgery Malaria |
Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2007, 282 (19), pp.14505-14514 Journal of Biological Chemistry, 2007, 282 (19), pp.14505-14514. ⟨10.1074/jbc.M608035200⟩ |
ISSN: | 0021-9258 1083-351X |
Popis: | Malaria is a major cause of morbidity and mortality with an annual death toll exceeding one million. Severe malaria is a complex multisystem disorder, including one or more of the following complications: cerebral malaria, anemia, acidosis, jaundice, respiratory distress, renal insufficiency, coagulation anomalies, and hyperparasitemia. Using a combined in vivo/in vitro metabolic-based approach, we investigated the putative pathogenic effects of Plasmodium berghei ANKA on brain, in a mouse strain developing malaria but resistant to cerebral malaria. The purpose was to determine whether the infection could cause a brain dysfunction distinct from the classic cerebral syndrome. Mice resistant to cerebral malaria were infected with P. berghei ANKA and explored during both the symptomless and the severe stage of the disease by using in vivo brain magnetic resonance imaging and spectroscopy. The infected mice did not present the lesional and metabolic hallmarks of cerebral malaria. However, brain dysfunction caused by anemia, parasite burden, and hepatic damage was evidenced. We report an increase in cerebral blood flow, a process allowing temporary maintenance of oxygen supply to brain despite anemia. Besides, we document metabolic anomalies affecting choline-derived compounds, myo-inositol, glutamine, glycine, and alanine. The choline decrease appears related to parasite proliferation. Glutamine, myo-inositol, glycine, and alanine variations together indicate a hepatic encephalopathy, a finding in agreement with the liver damage detected in mice, which is also a feature of the human disease. These results reveal the vulnerability of brain to malaria infection at the severe stage of the disease even in the absence of cerebral malaria. |
Databáze: | OpenAIRE |
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