Detection of electronegative low density lipoprotein (LDL−) in plasma and atherosclerotic lesions by monoclonal antibody-based immunoassays
| Autor: | Elaine Apolinário, Dulcinéia Saes Parra Abdalla, Joelma Maria Araujo de Oliveira, Alex Sevanian, Nágila Raquel Teixeira Damasceno, I. Fernandes |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Electronegative low-density lipoprotein Low-density lipoprotein receptor-related protein 8 medicine.drug_class Immunoblotting Clinical Biochemistry Antibody Affinity Aorta Thoracic Cross Reactions Monoclonal antibody Antigen Blood plasma medicine Humans Aged Aged 80 and over Immunoassay Chemistry Antibodies Monoclonal Cholesterol LDL General Medicine Middle Aged Atherosclerosis Chromatography Ion Exchange Molecular biology Immunization Immunohistochemistry Female lipids (amino acids peptides and proteins) Lipoprotein |
| Zdroj: | Clinical Biochemistry. 39:28-38 |
| ISSN: | 0009-9120 |
| DOI: | 10.1016/j.clinbiochem.2005.09.014 |
| Popis: | To produce a monoclonal antibody (MAb) against electronegative LDL (LDL-) for detecting this modified lipoprotein in blood plasma and tissues.LDL- was isolated from human blood plasma and used as an antigen for immunization of Balb/c mice. Lymphocytes of immunized mice were fused with myeloma cells (SP2/0) to obtain the hybridomas. LDL- was detected in blood plasma and atherosclerotic lesions of humans and rabbits by MAb-based ELISA and immunohistochemistry, respectively.LDL- concentrations were higher (P0.05) in the blood plasma of hypercholesterolemic subjects (HC, 248 +/- 77 mg/dL of total cholesterol) than in normolipidemic subjects (NL, 173 +/- 82 mg/dL of total cholesterol) and rabbits (HC, 250 +/- 15 mg/dL of cholesterol versus NL, 81 +/- 12 mg/dL of cholesterol). Moreover, LDL- was detected in the atherosclerotic lesions of humans and rabbits.These MAb-based immunoassays are adequate to detect LDL- in biological samples and represent an important tool for investigating the role of LDL- in atherosclerosis. |
| Databáze: | OpenAIRE |
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