Myocardial metabolism of pantothenic acid in chronically diabetic rats
| Autor: | Cathy J. Beinlich, James R. Neely, Won O. Song, Robert D. Naumovitz |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Male
medicine.medical_specialty Time Factors medicine.medical_treatment Tritium Pantothenic Acid Streptozocin Diabetes Mellitus Experimental In vivo Internal medicine Pantothenic acid Medicine Animals Carnitine Molecular Biology business.industry Insulin Myocardium Rats Inbred Strains Metabolism Streptozotocin Rats Endocrinology Cardiology and Cardiovascular Medicine business Perfusion Panthenol medicine.drug |
| Zdroj: | Journal of molecular and cellular cardiology. 22(3) |
| ISSN: | 0022-2828 |
| Popis: | Transport and metabolism of [3H]pantothenic acid ([3H]Pa) was investigated in hearts from control and streptozotocin-induced diabetic rats. In isolated perfused hearts from control animals, the transport of [3H]Pa was linear over 3 h of perfusion when 11 mM glucose was the only exogenous substrate. The in vitro transport of [3H]Pa by hearts from 48-h diabetic rats was reduced by 65% compared to controls and was linear over 2 h of perfusion with no further accumulation of Pa during the third hour. The defect in transport observed in vitro could be corrected by in vivo treatment with 4 U Lente insulin/day for 2 days. In vitro addition of insulin in the presence of 11 mM glucose or 11 mM glucose plus 1.2 mM palmitate had no effect on [3H]Pa transport in hearts from 48-h diabetic rats during 3 h of perfusion. Accumulation of [3H]Pa was not inhibited by inclusion of 0.7 mM amino acids, 1 mM carnitine, 50 microM mersalic acid or 1 mM panthenol, pantoyllactone or pantoyltaurine. Uptake was inhibited by 1 mM nonanoic, octanoic or heptanoic acid, 0.1 mM biotin or 0.25 mM probenecid, suggesting a requirement for the terminal carboxyl group for transport. Transport of pantothenic acid was reduced in hearts from diabetic rats within 24 h of injection of streptozotocin. In vitro accumulation of [3H]Pa decreased to 10% of control 1 week after streptozotocin injection and then remained at 30% of the control value over 10 weeks.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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