Alzheimer's Aβ fused to green fluorescent protein induces growth stress and a heat shock response
| Autor: | Ian Macreadie, Peter I. Macreadie, J.N. Varghese, Lynne J. Waddington, Helma Antony, Jo Caine, Sonia E. Sankovich |
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| Rok vydání: | 2007 |
| Předmět: |
Amyloid beta-Peptides
Recombinant Fusion Proteins Green Fluorescent Proteins Saccharomyces cerevisiae General Medicine Biology biology.organism_classification Applied Microbiology and Biotechnology Microbiology Peptide Fragments Green fluorescent protein Cell biology Biochemistry Protein folding Signal transduction Heat shock Lipid raft Heat-Shock Response Gamma secretase Intracellular |
| Zdroj: | FEMS Yeast Research. 7:1230-1236 |
| ISSN: | 1567-1364 1567-1356 |
| Popis: | The 42 amino acid Alzheimer's Abeta peptide is involved in the progression of Alzheimer's disease. Here we describe the effects of intracellular Abeta, produced through its attachment to either end of a green fluorescent protein, in yeast. Cells producing Abeta exhibited a lower growth yield and a heat shock response, showing that Abeta fusions promote stress in cells and supporting the notion that intracellular Abeta is a toxic molecule. These studies have relevance in understanding the role of Abeta in the death of neuronal cells, and indicate that yeast may be a new tractable model system for the screening for inhibitors of the stress caused by Abeta. |
| Databáze: | OpenAIRE |
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