Evaluation of the LDBio Aspergillus ICT lateral flow assay for serodiagnosis of allergic bronchopulmonary aspergillosis
| Autor: | Malcolm Richardson, Iain D. Page, David W. Denning, Elizabeth Stucky Hunter |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine Adolescent Science 030106 microbiology Immunoglobulin E Aspergillosis Aspergillus fumigatus Young Adult 03 medical and health sciences 0302 clinical medicine parasitic diseases medicine Humans Serologic Tests Antibodies Fungal Aged Retrospective Studies Asthma Aged 80 and over Immunoassay Multidisciplinary Bronchiectasis biology business.industry Chronic pulmonary aspergillosis Aspergillosis Allergic Bronchopulmonary Middle Aged biology.organism_classification medicine.disease United Kingdom Aspergillus Immunoglobulin M 030228 respiratory system Immunoglobulin G Immunology biology.protein Sputum Medicine Female Allergic bronchopulmonary aspergillosis medicine.symptom business |
| Zdroj: | PLoS ONE, Vol 15, Iss 9, p e0238855 (2020) Hunter, E S, Kniemeyer, O, Page, I D, Richardson, M D & Denning, D W 2020, ' Evaluation of the LDBio Aspergillus ICT lateral flow assay for serodiagnosis of allergic bronchopulmonary aspergillosis ', PLoS ONE, vol. 15, no. 9 . https://doi.org/10.1371/journal.pone.0238855 Hunter, E S, Kniemeyer, O, Page, I D, Richardson, M D & Denning, D W 2020, ' Evaluation of the LDBio Aspergillus ICT lateral flow assay for serodiagnosis of allergic bronchopulmonary aspergillosis ', P L o S One, vol. 15, no. 9 . https://doi.org/10.1371/journal.pone.0238855 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0238855 |
| Popis: | BackgroundEarly recognition and diagnosis of allergic bronchopulmonary aspergillosis (ABPA) is critical to improve patient symptoms, and antifungal therapy may prevent or delay progression of bronchiectasis and development of chronic pulmonary aspergillosis.ObjectiveA recently commercialized lateral flow assay (Aspergillus ICT) (LDBio Diagnostics, Lyons, France) detects Aspergillus-specific antibodies in MethodsABPA and control sera collected at the National Aspergillosis Centre (Manchester, UK) and/or from the Manchester Allergy, Respiratory and Thoracic Surgery research biobank were evaluated using the Aspergillus ICT assay. Results were read both visually and digitally (using a lateral flow reader). Serological Aspergillus-specific IgG and IgE, and total IgE titres were measured by ImmunoCAP.ResultsFor 106 cases of ABPA versus all diseased controls, sensitivity and specificity for the Aspergillus ICT were 90.6% and 87.2%, respectively. Sensitivity for 'proven' ABPA alone (n = 96) was 89.8%, and 94.4% for 'presumed' ABPA (n = 18). 'Asthma only' controls (no bronchiectasis) and 'bronchiectasis controls' exhibited 91.4% and 81.7% specificity, respectively. Comparison of Aspergillus ICT result with Aspergillus-specific IgG and IgE titres showed no evident immunoglobulin isotype bias. Digital measurements displayed no correlation between ImmunoCAP Aspergillus-specific IgE level and ICT test line intensity.ConclusionsThe Aspergillus ICT assay exhibits good sensitivity for ABPA serological screening. It is easy to perform and interpret, using minimal equipment and resources; and provides a valuable simple screening resource to rapidly distinguish more serious respiratory conditions from Aspergillus sensitization alone. |
| Databáze: | OpenAIRE |
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