203Hg labelled PHMB as reagent for the determination of –SH groups in native and denatured proteins by hydrophobic interaction chromatography
| Autor: | Giorgio Raspi, G. Tesi, Maria Spinetti, Emilia Bramanti |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Chemistry Hydrophilic interaction chromatography Aldolase A Dehydrogenase Biochemistry Analytical Chemistry Ovalbumin chemistry.chemical_compound Reagent Glyceraldehyde Electrochemistry biology.protein Thiol Environmental Chemistry Molecule Spectroscopy |
| Zdroj: | The Analyst. 124:511-515 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/a809381d |
| Popis: | Aldolase, glyceraldehyde 3-phosphate dehydrogenase and ovalbumin were determined by hydrophobic interaction chromatography, which allows the evaluation of the number of –SH groups per molecule of protein in both the native and denatured form. These proteins were chosen as models to show the generality of use of the procedure for analytical and biochemical applications. The experiments were performed by using 203Hg-labelled p-hydroxymercuribenzoate as reagent and known concentrations of proteins. The results were compared with FI-CV-ETAAS measurements and literature data. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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